Two non-mammalian toll-like receptors (TLR21 and TLR22) from golden pompano (Trachinotus ovatus): Molecular cloning, gene characterization and expression analysis
Two non-mammalian toll-like receptors (TLR21 and TLR22) from golden pompano (Trachinotus ovatus): Molecular cloning, gene characterization and expression analysis
复制标题
来自金鲳鱼（Trachinotus ovatus）的两种非哺乳动物 Toll 样受体（TLR21 和 TLR22）：分子克隆、基因表征和表达分析
DOI：
10.1016/j.aqrep.2021.100912
复制
发表时间：
2021-11
影响因子：
3.7
通讯作者：
Zhitao Qi
中科院分区：
文献类型：
--
作者：
Shuangshuang Gao;Teng Xu;Ruifeng Qiao;Jianchao Lu;Yang Xu;Shu Hu;Youchuan Wei;Zhitao Qi
Toll样受体（TLR）是重要的模式识别受体（PRR），参与识别病原体相关分子模式（PAMPs），在宿主先天免疫中发挥关键作用。在本研究中，两个非哺乳动物的TLRs，troTLR 21和troTLR 22，鉴定和特征的黄金鲳，具有巨大的经济价值的海洋硬骨鱼。这两个TLRs均为典型的TLRs，含有TLRs家族中保守的多个基序，包括富含亮氨酸重复序列（LRR）基序、跨膜结构域和Toll/白细胞介素1受体（TIR）结构域。这两个TLRs与其他硬骨鱼类中的TLRs具有很高的序列同源性。系统进化树分析表明，troTLR 21和troTLR 22分别与其它鱼类TLR 21和TLR 22聚类较好。实时荧光定量PCR（qPCR）结果显示，这两种TLR在所有研究组织中都有广泛表达，在免疫相关组织（脾和头肾（HK））中转录水平较高。此外，troTLR 21和troTLR 22在溶藻弧菌、脂多糖（LPS）和聚肌胞苷酸（polyI：C）刺激后在HK和脾脏中的表达显著上调。以上结果提示这两种TLRs参与了金鲳抵御病原入侵的免疫应答，为进一步研究鱼类特异性TLRs的功能奠定了基础。
Toll-like receptors (TLRs), important pattern recognition receptors (PRRs), involve in recognizing pathogen-associated molecular patterns (PAMPs) and play crucial roles in the host innate immunity. In the present study, two non-mammalian TLRs, troTLR21 and troTLR22, were identified and characterized from golden pompano (Trachinotus ovatus), a marine teleost with great economic values. The two TLRs were typical TLRs, containing several motifs conserved in TLRs family including leucine-rich repeat (LRR) motifs, a transmembrane domain, and a toll/interleukin-1 receptor (TIR) domain. The two TLRs shared high sequence identity with their counterparts in other teleosts. Phylogenetic tree analysis showed that troTLR21 and troTLR22 were well clustered with other fish TLR21 and TLR22, respectively. Quantitative real-time PCR (qPCR) revealed that the two TLRs were ubiquitously expressed in all investigated tissues, with higher transcription levels in the immune-related tissues (spleen and head kidney (HK)). Further, the expressions of troTLR21 and troTLR22 were significantly up-regulated in HK and spleen followingVibrio alginolyticus, lipopolysaccharide (LPS), and polyinosinic: polycytidylic acid (polyI:C) stimulation. Our results suggested that the two TLRs involve in the immune response against pathogen invasion in golden pompano, providing solid basis for further study the functions of fish-specific TLRs.
登录
查看更多内容
金鲳卵形鲳鲳弧菌的分离、鉴定及致病性研究
影响因子：
4.5
作者：
Liu Shubin;Li Erchao;Cai Yan;Wang Shifeng;Ren Zhuling;Li Qiming;Guo Weiliang;Wu Yue;Zhou Yongcan
通讯作者：
Zhou Yongcan
影响因子：
3
作者：
M. Samanta;B. Swain;M. Basu;G. Mahapatra;Bikash R. Sahoo;Mahismita Paichha;S. Lenka;P. Jayasankar
通讯作者：
M. Samanta;B. Swain;M. Basu;G. Mahapatra;Bikash R. Sahoo;Mahismita Paichha;S. Lenka;P. Jayasankar
影响因子：
4.7
作者：
Hu, Guo-Bin;Zhang, Shou-Feng;Zhang, Shi-Cui
通讯作者：
Zhang, Shi-Cui
影响因子：
4.7
作者：
J. Xing;Xiujuan Zhou;Xiaoqian Tang;X. Sheng;W. Zhan
通讯作者：
J. Xing;Xiujuan Zhou;Xiaoqian Tang;X. Sheng;W. Zhan
影响因子：
4.7
作者：
Qi, Zhitao;Wang, Sisi;Wei, Qiwei
通讯作者：
Wei, Qiwei