Oxidation of nitroxide radicals by the reaction of hemoglobin with hydrogen peroxide.
Oxidation of nitroxide radicals by the reaction of hemoglobin with hydrogen peroxide.
复制标题
通过血红蛋白与过氧化氢的反应氧化硝基氧自由基。
DOI：
10.1016/0006-291x(84)91287-7
复制
发表时间：
1984
影响因子：
3.1
通讯作者：
E. Kimoto
中科院分区：
文献类型：
--
作者：
T. Yamaguchi;T. Nakano;E. Kimoto
过氧化氢的加入使血红蛋白溶液中的4-羟基-羟基-2,2,6,6-四甲基哌啶的ESR信号明显降低。离子交换色谱和四苯基硼酸钠对反应溶液的反应结果表明，氮氧自由基被氧化成阳离子形式。通过薄层色谱与硝基自由基与HCl或Br2的反应产物的对比，证实了4-羟基- 1 -氧-2,2,6,6-四甲基哌替啶阳离子的形成。这一结果得到了13c核磁共振测量的支持。
The ESR signal of 4-hydroxy-l-oxyl-2,2,6,6-tetramethylpiperidine in hemoglobin solution decreased drastically by the addition of hydrogen peroxide. The results of ion-exchange chromatography and sodium tetraphenylborate on the reaction solution showed an oxidation of the nitroxide radical to cation form. On the basis of the comparison of thin layer-chromatogram with the reaction products of the nitroxide radicals with HCl or Br2, the formation of 4-hydroxy-l-oxo-2,2,6,6-tetramethylpiperidinium cation was demonstrated. This result was supported by the13C NMR measurement.
DOI：
10.1016/s0021-9258(17)44585-6
发表时间：
1983-08
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
D. J. Porter;H. Bright
通讯作者：
D. J. Porter;H. Bright