Antisense Inhibition of the Iron-Sulphur Subunit of Succinate Dehydrogenase Enhances Photosynthesis and Growth in Tomato via an Organic Acid-Mediated Effect on Stomatal Aperture
Antisense Inhibition of the Iron-Sulphur Subunit of Succinate Dehydrogenase Enhances Photosynthesis and Growth in Tomato via an Organic Acid-Mediated Effect on Stomatal Aperture
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DOI：
10.1105/tpc.110.081224
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发表时间：
2011-02-01
期刊：
影响因子：
11.6
通讯作者：
Fernie, Alisdair R.
中科院分区：
文献类型：
--
作者：
Araujo, Wagner L.;Nunes-Nesi, Adriano;Fernie, Alisdair R.
在35 S启动子的控制下以反义方向表达编码琥珀酸脱氢酶蛋白复合物的铁硫亚基的S1 SDH 2 -2基因片段的转基因番茄（Solanum lycopersicum）植物表现出增强的光合作用速率。在这些转化体中，三羧酸（TCA）循环的速率降低，并且与TCA循环相关的代谢物水平发生变化。此外，与野生型植物相比，在环境条件下，转基因植物中的二氧化碳同化作用增强了25%，成熟植物的特征在于生物量增加。附加光合参数分析表明，转基因植株的蒸腾速率和气孔导度显著升高。转化体表现出强烈增强的同化率在环境和次优环境条件下，以及升高的最大气孔开度。相反，当S1 SDH 2 -2基因以保卫细胞特异性方式被反义RNA抑制时，观察到气孔孔径和光合作用都没有变化。所获得的数据进行了讨论的TCA循环中间体的作用的背景下，一般相对于光合代谢，特别是相对于它们的作用，在调节气孔开度。
Transgenic tomato (Solanum lycopersicum) plants expressing a fragment of the Sl SDH2-2 gene encoding the iron sulfur subunit of the succinate dehydrogenase protein complex in the antisense orientation under the control of the 35S promoter exhibit an enhanced rate of photosynthesis. The rate of the tricarboxylic acid (TCA) cycle was reduced in these transformants, and there were changes in the levels of metabolites associated with the TCA cycle. Furthermore, in comparison to wild-type plants, carbon dioxide assimilation was enhanced by up to 25% in the transgenic plants under ambient conditions, and mature plants were characterized by an increased biomass. Analysis of additional photosynthetic parameters revealed that the rate of transpiration and stomatal conductance were markedly elevated in the transgenic plants. The transformants displayed a strongly enhanced assimilation rate under both ambient and suboptimal environmental conditions, as well as an elevated maximal stomatal aperture. By contrast, when the Sl SDH2-2 gene was repressed by antisense RNA in a guard cell-specific manner, changes in neither stomatal aperture nor photosynthesis were observed. The data obtained are discussed in the context of the role of TCA cycle intermediates both generally with respect to photosynthetic metabolism and specifically with respect to their role in the regulation of stomatal aperture.