D(TG)N.D(CA)N SEQUENCES UPSTREAM OF THE RAT PROLACTIN GENE FORM Z-DNA AND INHIBIT GENE-TRANSCRIPTION
D(TG)N.D(CA)N SEQUENCES UPSTREAM OF THE RAT PROLACTIN GENE FORM Z-DNA AND INHIBIT GENE-TRANSCRIPTION
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DOI：
10.1093/nar/18.6.1595
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发表时间：
1990-03-25
影响因子：
14.9
通讯作者：
CLARK, EM
中科院分区：
文献类型：
--
作者：
NAYLOR, LH;CLARK, EM
Two alternating purine-pyrimidine sequences of the d(TG)n .cntdot. d(CA)n-type (170bp and 60 bp in length) lie upstream of the rat prolactin (rPRL) gene. Conformational studies of plasmids containing these sequences indicate that both form left-handed (Z) DNA, with transitions initiating at superhelical densities of -0.041 and -0.044 respectively. These alternating purine-pyrimidine (APP) sequences are hypersensitive to cleavage with S1 nuclease both at the boundaries and within these APP repeats, where there is a loss in APP alternation. We have investigated that function of one of these Z-DNA sequences in the regulation of rPRL transcription, by linking regions of the 5'' flanking sequence of the rPRL gene to a reporter gene encoding chloramphenicol acetyltransferase (CAT), and transferring these plasmids into GH3 pituitary tumour cell lines. The major conclusion for these studies is that the 170bp repeat exerts a negative effect on the transcription of the rPRL gene, and also down-regulates the expression of the fusion gene pRSVcat when cloned 50bp upstream of the Rous sarcoma virus promoter. However, despite its proximity to an estrogen response element in prolactin, this sequence does not affect the responsiveness of the rPRL gene to estrogen.
