AVP neurons act as the primary circadian pacesetter cells in vivo
AVP neurons act as the primary circadian pacesetter cells in vivo
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DOI：
10.1101/2022.08.04.502742
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发表时间：
2022-08
期刊：
影响因子：
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通讯作者：
Y. Tsuno;Yubo Peng;Shin-ichi Horike;K. Yamagata;Mizuki Sugiyama;Takahiro J. Nakamura;T. Daikoku;T. Maejima;Michihiro Mieda
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文献类型：
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作者：
Y. Tsuno;Yubo Peng;Shin-ichi Horike;K. Yamagata;Mizuki Sugiyama;Takahiro J. Nakamura;T. Daikoku;T. Maejima;Michihiro Mieda
视交叉上核（SCN）的中央生物钟是一个由各种神经元和神经胶质细胞组成的网络。单个细胞具有细胞时钟的自主分子机制，但它们的固有周期变化很大。在这里，我们发现精氨酸加压素（AVP）神经元设置 SCN 网络的整体周期来控制昼夜行为节律。通过删除整个 SCN 中的酪蛋白激酶 1 δ (CK1δ) 来人工延长细胞周期，可以延长行为节律的自由运行周期，其程度类似于 AVP 神经元特有的 CK1δ 删除。在 SCN 切片中，这些小鼠的 PER2::LUC 报告基因节律没有重现周期延长。然而，AVP 和血管活性肠肽 (VIP) 神经元的体内钙节律均表现出与 AVP 神经元特异性 CK1δ 缺失后的行为节律相似的延长周期。这些结果表明 AVP 神经元是 SCN 系综周期的主要决定因素。
The central circadian clock of the suprachiasmatic nucleus (SCN) is a network consisting of various neurons and glia. Individual cells have the autonomous molecular machinery of a cellular clock, but their intrinsic periods are considerably variable. Here, we show that arginine vasopressin (AVP) neurons set the ensemble period of the SCN network to control circadian behavior rhythm. Artificial lengthening of cellular periods by deleting casein kinase 1 delta (CK1δ) in the whole SCN lengthened the free-running period of behavior rhythm to an extent similar to CK1δ deletion specific to AVP neurons. In SCN slices, PER2::LUC reporter rhythms of these mice did not recapitulate the period lengthening. However, in vivo calcium rhythms of both AVP and vasoactive intestinal peptide (VIP) neurons demonstrated lengthened periods similar to the behavioral rhythm upon AVP neuron-specific CK1δ deletion. These results indicate that AVP neurons act as the primary determinant of the SCN ensemble period.