Improved detection of an alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus
Improved detection of an alien invasive species through environmental DNA barcoding: the example of the American bullfrog Lithobates catesbeianus
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DOI：
10.1111/j.1365-2664.2012.02171.x
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发表时间：
2012-08-01
影响因子：
5.7
通讯作者：
Miaud, Claude
中科院分区：
文献类型：
--
作者：
Dejean, Tony;Valentini, Alice;Miaud, Claude
1.外来入侵物种是造成生物多样性丧失和全球同质化的主要原因之一。一旦建立了人工免疫系统，控制成本可能会非常高，而且并不总是能够完全根除。在低密度下发现物种的能力大大提高了根除的成功率，并降低了控制成本和对生态系统的影响。2.在本研究中，我们比较了基于听觉和视觉接触调查的传统现场方法与环境DNA(EDNA)调查方法在检测入侵法国西南部的美国牛蛙Rana catesbeiana=Lithobates catesbeianus方面的敏感性。3.论证了EDNA方法在物种检测中的应用价值，并在灵敏度和采样量方面均优于传统的两栖动物调查方法。使用分子方法在38个地点发现了牛蛙，而使用昼夜调查发现了7个地点，这表明传统的野外调查严重低估了美国牛蛙的分布。4.合成与应用。环境DNA方法允许在极低密度和任何生命阶段及早检测外来入侵物种，这对于检测稀有和/或隐秘的水生物种特别重要。这种方法还可以用来确认控制行动的敏感性，更好地确定脆弱物种的分布，使其成为物种清查和管理的一个非常相关的工具。
1. Alien invasive species (AIS) are one of the major causes of biodiversity loss and global homogenization. Once an AIS becomes established, costs of control can be extremely high and complete eradication is not always achieved. The ability to detect a species at a low density greatly improves the success of eradication and decreases both the costs of control and the impact on ecosystems. 2. In this study, we compare the sensitivity of traditional field methods, based on auditory and visual encounter surveys, with an environmental DNA (eDNA) survey for the detection of the American bullfrog Rana catesbeiana = Lithobates catesbeianus, which is invasive in south-western France. 3. We demonstrate that the eDNA method is valuable for species detection and surpasses traditional amphibian survey methods in terms of sensitivity and sampling effort. The bullfrog was detected in 38 sites using the molecular method, compared with seven sites using the diurnal and nocturnal surveys, suggesting that traditional field surveys have strongly underestimated the distribution of the American bullfrog. 4. Synthesis and applications. The environmental DNA approach permits the early detection of alien invasive species (AIS), at very low densities and at any life stage, which is particularly important for the detection of rare and/or secretive aquatic species. This method can also be used to confirm the sensitivity of control operations and to better identify the distributions of vulnerable species, making this a very relevant tool for species inventory and management.