Cloning of rhesus monkey killer-cell Ig-like receptors (KIRs) from early pregnancy decidua
Cloning of rhesus monkey killer-cell Ig-like receptors (KIRs) from early pregnancy decidua
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DOI：
10.1034/j.1399-0039.2001.580507.x
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发表时间：
2001-11-01
期刊：
影响因子：
--
通讯作者：
Golos, TG
中科院分区：
文献类型：
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作者：
Grendell, RL;Hughes, AL;Golos, TG
为了确定恒河猴蜕膜内表达的免疫细胞Ig样受体（KIR）分子，用位于KIR D2结构域内的寡核苷酸筛选早孕恒河猴蜕膜文库。我们获得了26个代表II mRNA的全长克隆。cDNA编码含有2或3个IG结构域和短或长胞质结构域的蛋白质。长结构域含有免疫酪氨酸抑制基序（ITIM）基序，表明抑制功能。三个克隆在E11结构域内含有单或二核苷酸缺失，这将导致蛋白质在该结构域内截短。IG结构域的系统发育分析支持恒河猴KIR 2DL 4.1的D 0- D2组织，类似于人KIR 2DL 4和KIR 2DL 5。恒河猴KIR2DL4.1转录物含有与人KIR 2DL 4同源的长胞质尾。
To define the killer-cell Ig-like receptors (KIR) molecules expressed within the decidua in the rhesus monkey, an early pregnancy rhesus decidual library was screened with an oligonucleotide located within the KIR D2 domain. We obtained 26 full-length clones which represented I I mRNAs. The cDNAs encode proteins containing 2 or 3 Ig domains, and short or long cytoplasmic domains. The long domains contained immunotyrosine inhibitory motifs (ITIM) motifs indicating inhibitory function. Three clones contained single or dinucleotide deletions within the Ell domain, which would result in proteins truncated Within this domain. Phylo-genetic analysis of the Ig domains supports a D0 - D2 organization of rhesus KIR2DL4.1, similar to human KIR2DL4 and KIR2DL5. The rhesus, KIR2DL4.1 transcript contains a long cytoplasmic tail homologous with human KIR2DL4.