Detection of misidentifications of species from the Burkholderia cepacia complex and description of a new member, the soil bacterium Burkholderia catarinensis sp nov.
Detection of misidentifications of species from the Burkholderia cepacia complex and description of a new member, the soil bacterium Burkholderia catarinensis sp nov.
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DOI：
10.1093/femspd/ftx076
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发表时间：
2017-08-01
影响因子：
3.3
通讯作者：
Pereira Passaglia, Luciane Maria
中科院分区：
文献类型：
--
作者：
Bach, Evelise;Sant'Anna, Fernando Hayashi;Pereira Passaglia, Luciane Maria
正确鉴定洋葱伯克霍尔德菌复合体（Bcc）的细菌对流行病学研究和囊性纤维化感染的治疗至关重要。然而，基于基因组的鉴定工具揭示了许多有争议的Bcc物种分配。本研究的目的是通过多相和基因组学方法重新研究土壤细菌B. cepacia 89的分类位置。recA和16S rRNA基因序列分析将菌株89定位在Bcc组内。然而，基于7个串联等位基因序列的差异评分、平均核苷酸同一性值和数字DNA: DNA杂交，我们的结果表明菌株89与以前描述的其他Bcc物种不同。因此，我们建议以菌株89(T) (=DSM 103188（T) = BR 10601(T)）为型菌株，将Burkholderia sp. 89分类为新种Burkholderia catarinensis sp. 11 .。此外，我们的研究结果引起了人们对国家生物技术信息中心数据库中Bcc基因组的一些可能的错误识别的关注。
The correct identification of bacteria from the Burkholderia cepacia complex (Bcc) is crucial for epidemiological studies and treatment of cystic fibrosis infections. However, genome-based identification tools are revealing many controversial Bcc species assignments. The aim of this work is to re-examine the taxonomic position of the soil bacterium B. cepacia 89 through polyphasic and genomic approaches. recA and 16S rRNA gene sequence analysis positioned strain 89 inside the Bcc group. However, based on the divergence score of seven concatenated allele sequences, and values of average nucleotide identity, and digital DNA: DNA hybridization, our results suggest that strain 89 is different from other Bcc species formerly described. Thus, we propose to classify Burkholderia sp. 89 as the novel species Burkholderia catarinensis sp. nov. with strain 89(T) (=DSM 103188(T) = BR 10601(T)) as the type strain. Moreover, our results call the attention to some probable misidentifications of Bcc genomes at the National Center for Biotechnology Information database.