CHARACTERIZATION OF ENTEROADHERENT-AGGREGATIVE ESCHERICHIA-COLI, A PUTATIVE AGENT OF DIARRHEAL DISEASE
CHARACTERIZATION OF ENTEROADHERENT-AGGREGATIVE ESCHERICHIA-COLI, A PUTATIVE AGENT OF DIARRHEAL DISEASE
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DOI：
10.1093/infdis/158.1.70
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发表时间：
1988-07-01
影响因子：
6.4
通讯作者：
LEVINE, MM
中科院分区：
文献类型：
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作者：
VIAL, PA;ROBINSBROWNE, R;LEVINE, MM
显示HEp-2细胞粘附的聚集模式的大肠杆菌（肠粘附-聚集性大肠杆菌（enteroadherent-aggregative E.大肠杆菌[EA-AggEC]）在流行病学上被认为是腹泻的原因。我们进行了初步的微生物和致病特性的42株这种假定的病原体。用肠致病性、肠致病性、肠侵袭性和肠出血性E.大肠杆菌，血清型，不符合这些类别。42个菌株中有39个具有55-65兆道尔顿的质粒;许多共享DNA同源性。对于一个代表性菌株，质粒转移伴随着光滑脂多糖、菌毛表达和聚集特性的转移。EA-AggEC引起兔和大鼠回肠袢的特征性病变。接种活生物体的兔子的肠道病变和（志贺样）肢体麻痹和死亡表明与毒素有关;志贺样毒素检测呈阴性。这些初步结果支持了EA-AggEC可能代表一种独特的大肠杆菌的论点。杆菌
Escherichia coli that exhibit the aggregative pattern of adherence of HEp-2 cells (enteroadherent-aggregative E. coli [EA-AggEC]) have been epidemiologically incriminated as a cause of diarrhea. We undertook a preliminary microbiological and pathogenetic characterization of 42 isolates of this putative pathogen. The strains were negative by tests with DNA probes for enteropathogenic, enterotoxigenic, enteroinvasive, and enterohemorrhagic E. coli and, by serotype, did not fit these categories. Thirty-nine of 42 strains had a 55-65-megadalton plasmid; many shared DNA homology. With one representative strain, plasmid transfer was accompanied by transfer of smooth lipopolysaccharide, fimbriae expression, and the aggregative property. EA-AggEC caused characteristic lesions in rabbit and rat ileal loops. The intestinal lesions and (Shiga-like) limb paralysis and death in rabbits inoculated with live organisms suggest toxin involvement; assays for Shiga-like toxins were negative. These preliminary results support the contention that EA-AggEC may represent a distinct category of diarrheagenic E. coli.