Single cell transcriptomic landscapes of pattern formation, proliferation and growth in Drosophila wing imaginal discs
Single cell transcriptomic landscapes of pattern formation, proliferation and growth in Drosophila wing imaginal discs
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DOI：
10.1242/dev.179754
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发表时间：
2019-09-01
期刊：
影响因子：
4.6
通讯作者：
Yan, Yan
中科院分区：
文献类型：
--
作者：
Deng, Mingxi;Wang, Ying;Yan, Yan
器官形成依赖于发育过程中模式形成、增殖和生长的协调。这些过程如何在单个细胞水平上整合仍不清楚。在过去的几十年里，以果蝇翅盘作为模型系统的研究为模式形成、生长控制和再生提供了有价值的见解。在这里，我们提供了单细胞转录景观的模式形成，增殖和增长的翼成虫盘。我们发现，图案化信息是鲁棒地保持在单细胞转录组数据，并可以提供参考矩阵的计算映射到离散的空间域的单细胞。分配翅盘单细胞的空间亚区有利于图案细化过程的检查。我们还将单个细胞聚类为不同的增殖和生长状态，并评估了细胞增殖/生长状态与空间模式之间的相关性。此外，单细胞转录组学分析使我们能够定量检查在良好建立的肿瘤模型中分化、增殖和生长的干扰。我们在www.example.com上提供了一个数据库来探索这些数据集http://drosophilayanlab-virtual-wingdisc.ust.hk:3838/v2/.This文章有一个相关的论文采访背后的人。
Organ formation relies on the orchestration of pattern formation, proliferation and growth during development. How these processes are integrated at the individual cell level remains unclear. In the past decades, studies using Drosophila wing imaginal discs as a model system have provided valuable insights into pattern formation, growth control and regeneration. Here, we provide single cell transcriptomic landscapes of pattern formation, proliferation and growth of wing imaginal discs. We found that patterning information is robustly maintained in the single cell transcriptomic data and can provide reference matrices for computationally mapping single cells into discrete spatial domains. Assignment of wing disc single cells to spatial subregions facilitates examination of patterning refinement processes. We also clustered single cells into different proliferation and growth states and evaluated the correlation between cell proliferation/growth states and spatial patterning. Furthermore, single cell transcriptomic analyses allowed us to quantitatively examine disturbances of differentiation, proliferation and growth in a well-established tumor model. We provide a database to explore these datasets at http://drosophilayanlab-virtual-wingdisc.ust.hk:3838/v2/.This article has an associated The people behind the papers' interview.