MiR-34a-Functionalized Hydroxyapatite by Lyophilization Promoted Bone Regeneration in Irradiated Bone Defects
MiR-34a-Functionalized Hydroxyapatite by Lyophilization Promoted Bone Regeneration in Irradiated Bone Defects
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DOI：
10.1155/2023/9946012
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发表时间：
2023-09-11
影响因子：
3.3
通讯作者：
Liu，Huan
中科院分区：
文献类型：
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作者：
Wu，Xi;Feng，Xiaoke;Liu，Huan
放射治疗后骨缺损的修复需要骨诱导替代物的开发。MicroRNA可作为一种成骨因子，用于构建骨再生功能材料。在本研究中，我们使用miR-34a来促进照射后的骨再生。我们将脂联胺-agomiR-34a脂合物冷冻干燥到羟基磷灰石(HA)上，制备了miR-34a功能化羟基磷灰石(HA-agomiR-34a)。用扫描电子显微镜和原子力显微镜观察了其形貌。荧光显微镜证实了agomiR-34a在HA表面的滞留。HA-agomiR-34a具有较高的转染率和良好的生物相容性。HA-agomiR-34a促进辐射损伤的骨髓基质细胞向成骨细胞分化。植入HA-agomiR-34a可促进放射性骨缺损区的骨再生。HA-agomiR-34a可能是一种新型的、安全的骨替代材料，可促进放疗后骨缺损的修复。
The rehabilitation of bone defects after radiotherapy requires the development of osteoinductive bone substitutes. MicroRNA could be used as an osteogenic factor to fabricate functional materials for bone regeneration. In this study, we used miR‐34a to enhance bone regeneration after irradiation. We lyophilized lipofectamine‐agomiR‐34a lipoplexes on hydroxyapatite (HA) to develop miR‐34a‐functionalized hydroxyapatite (HA‐agomiR‐34a). The morphology was observed by scanning electron microscope and atomic force microscope. Fluorescence microscopy confirmed the retention of agomiR‐34a on the surface of HA. HA‐agomiR‐34a showed high transfection efficiency and good biocompatibility. HA‐agomiR‐34a enhanced the osteoblastic differentiation of radiation‐impaired bone marrow stromal cells (BMSCs). Implantation of HA‐agomiR‐34a promoted bone regeneration in irradiated bone defects. HA‐agomiR‐34a may be a novel and safe bone substitute to promote the reconstruction of bone defects after radiotherapy.