A comparative analysis of the structural architecture of ssDNA viruses
A comparative analysis of the structural architecture of ssDNA viruses
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DOI：
10.1080/17486700802168247
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发表时间：
2008-01-01
影响因子：
--
通讯作者：
Agbandje-McKenna, Mavis
中科院分区：
文献类型：
--
作者：
Bennett, Antonette;McKenna, Robert;Agbandje-McKenna, Mavis
利用有限数量的病毒外壳蛋白（CP或VP）构建单元的病毒组装是自然界中发生的定向大分子相互作用的一个很好的例子。球形（二十面体）病毒的组装有两个基本原则：（i）遗传经济--衣壳化的基因组编码一个或几个CP，它们组装成一个保护壳（病毒衣壳）;（ii）特异性--CP必须折叠以相互识别，并在组装过程中形成精确的CP-CP界面相互作用。利用各种生物物理技术，包括X射线晶体学和冷冻电子显微镜，结合同源模型的建立，生物化学和分子生物学，蛋白质-蛋白质亚基和蛋白质-核酸之间的相互作用，促进病毒衣壳装配的性质进行了研究。这篇综述讨论了微病毒科，双生病毒科和细小病毒科的ssDNA的相似性和差异，已阐明。微病毒科代表噬菌体家族，其利用几种CP和支架蛋白来组装T = 1二十面体衣壳，双生病毒科植物病毒装配一个独特孪生准等轴由单个CP组装的（成对的）假T = 1病毒粒子，细小病毒科代表动物病毒，其T = 1衣壳由具有独特N-末端的2 - 4个VP的共同重叠区域形成。终端扩展对这些病毒可用的三维（3D）数据的调查显示，它们利用结构共性，由不同的CP/VP氨基酸序列促进成熟感染性病毒体的成功组装。
Virus assembly, utilizing a limited number of viral coat protein (CP or VP) building blocks, is an excellent example of a directed macromolecular interaction occurring in nature. Two basic principles govern the assembly of spherical (icosahedral) viruses: (i) Genetic Economy - the encapsidated genome encodes a single or few CPs that assemble a protective shell (the viral capsid); and (ii) specificity - the CPs have to fold to recognize each other and form exact CP-CP interfacial interactions during the assembly pathway. Using a variety of biophysical techniques, including X-ray crystallography and cryo-electron microscopy, combined with homology model building, biochemistry and molecular biology, the nature of the interactions between protein-protein subunits and protein-nucleic acid that facilitate viral capsid assembly have been studied. This review discusses both the similarities and differences that have been elucidated for ssDNA Microviridae, Geminiviridae, and Parvoviridae virus families. The Microviridae represent a family of bacteriophages that utilize several CPs and scaffold proteins to assemble a T = 1 icosahedral capsid, the Geminiviridae plant viruses assemble a unique twinned quasi-isometric (geminate) pseudo T = 1 virion assembled from a single CP and the Parvoviridae represent animal viruses whose T = 1 capsids are formed from the common overlapping region of two to four VPs that have unique N-terminal extensions. A survey of the three-dimensional (3D) data available for these viruses shows that they utilize structural commonalities, facilitated by disparate CP/VP amino acid sequences for the successful assembly of mature infectious virions.