Comparison of sesamin contents and CYP81Q1 gene expressions in aboveground vegetative organs between two Japanese sesame (Sesamum indicum L.) varieties differing in seed sesamin contents
Comparison of sesamin contents and CYP81Q1 gene expressions in aboveground vegetative organs between two Japanese sesame (Sesamum indicum L.) varieties differing in seed sesamin contents
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DOI：
10.1016/j.plantsci.2010.02.020
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发表时间：
2010-06-01
期刊：
影响因子：
5.2
通讯作者：
Kobayashi, Akio
中科院分区：
文献类型：
--
作者：
Hata, Naoki;Hayashi, Yoshinori;Kobayashi, Akio
芝麻属植物的叶子在尼日利亚和世界许多热带地区被用作叶类蔬菜。芝麻(芝麻)叶在日本也被用作一种含有功能成分的新食品原料。芝麻素是芝麻籽的主要木脂素成分，被认为对人体有许多有益的健康影响，但以前从未在芝麻叶中检测到。建立了芝麻叶片中芝麻素的超高效液相色谱-荧光检测法(UPLC-FLD)分析方法，研究了芝麻素含量不同的两个日本芝麻品种(芝麻素含量分别为15和5mgg(-1)干重)的基因型差异。我们的方法首次实现了芝麻叶中芝麻素的定量。播种5周后，‘戈马豆’和‘京玛’叶片的芝麻素含量分别为2.6和0.5mgg(-1)DW，约为种子芝麻素含量的1/5000或更低。‘戈马豆’的芝麻素含量较高，说明叶片的芝麻素含量与种子的芝麻素含量相关。芝麻素生物合成基因CYP81Q1在‘戈马豆’中的表达显著高于‘亲戈马’，表明该基因的表达差异是影响叶片芝麻素含量的重要因素之一。(C)2010爱思唯尔爱尔兰有限公司。保留所有权利。
Leaves of Sesamum spp. are used as leafy vegetables in Nigeria and many tropical areas around the world. Sesame (Sesamum indicum) leaves are also being used in japan as a new food material containing functional components. Sesamin, a major lignan constituent of sesame seed, is considered responsible for a number of beneficial health effects in humans, but has not previously been detected in sesame leaves. We established an analytical method of detecting sesamin in sesame leaves using ultra performance liquid chromatography-fluorescence detection (UPLC-FLD), and investigated genotypic differences in sesamin content of two Japanese sesame varieties that differ in seed sesamin content (15 and 5 mg g(-1) DW in 'Gomazou' and 'Kin-goma', respectively, quantified by the established method). Our method enabled quantification of sesamin in sesame leaves for the first time. The sesamin content of 'Gomazou' and 'Kingoma' leaves at 5 weeks after sowing was 2.6 and 0.5 mu g g(-1) DW, respectively, which is approximately 1/5000 or less of the sesamin content of seeds. The higher sesamin content of 'Gomazou' suggests that sesamin content of leaves correlates with that of seeds. The expression of the sesamin biosynthetic gene CYP81Q1 was considerably higher in 'Gomazou' than in 'Kin-goma', indicating that genotypic difference of CYP81Q1 gene expression is one of the important factors affecting leaf sesamin contents. (C) 2010 Elsevier Ireland Ltd. All rights reserved.