Approaches to measure the fitness of Burkholderia cepacia complex isolates
Approaches to measure the fitness of Burkholderia cepacia complex isolates
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DOI：
10.1099/jmm.0.017830-0
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发表时间：
2010-06-01
影响因子：
3
通讯作者：
McHugh, T. D.
中科院分区：
文献类型：
--
作者：
Pope, C. F.;Gillespie, S. H.;McHugh, T. D.
洋葱伯克霍尔德菌复合体（Bcc）的成员对许多抗菌剂具有高度耐药性，感染可能难以根除。使用与Bcc生长和生存条件相关的方法，采用协调的方法来测量从囊性纤维化（CF）慢性Bcc感染患者中分离出的Bcc细菌的适合度。在分离株中观察到生长速度的差异，生长速度较慢的分离株具有较高的mic和对更多抗菌素类的耐药性。测定了gyrA喹诺酮类耐药区核苷酸序列，发现环丙沙星MIC与密码子83和87的氨基酸替换相关。生物学相关的适应度测量方法被开发出来，并可用于调查更多的临床分离株。我们还描述了一种测定Bcc细菌突变率的方法。与铜绿假单胞菌不同的是，从CF患者分离的菌株中检测到高变变性，我们无法证明在这组新绿伯克氏菌和多沃氏伯克氏菌分离株中存在高变变性。
Members of the Burkholderia cepacia complex (Bcc) are highly resistant to many antibacterial agents and infection can be difficult to eradicate. A coordinated approach has been used to measure the fitness of Bcc bacteria isolated from cystic fibrosis (CF) patients with chronic Bcc infection using methods relevant to Bcc growth and survival conditions Significant differences in growth rate were observed among isolates, slower growth rates were associated with isolates that exhibited higher MICs and were resistant to more antimicrobial classes. The nucleotide sequences of the quinolone resistance-determining region of gyrA in the isolates were determined and the ciprofloxacin MIC correlated with amino acid substitutions at codons 83 and 87. Biologically relevant methods for fitness measurement were developed and could be applied to investigate larger numbers of clinical isolates. These methods were determination of planktonic growth rate, biofilm formation, survival in water and survival during drying We also describe a method to determine mutation rate in Bcc bacteria Unlike in Pseudomonas aeruginosa where hypermutability has been detected in strains isolated from CF patients, we were unable to demonstrate hypermutability in this panel of Burkholderia cenocepacia and Burkholderia multivorans isolates.