Effects of dietary oil sources and fat extraction methods on apparent and standardized ileal digestibility of fat and fatty acids in growing pigs.
Effects of dietary oil sources and fat extraction methods on apparent and standardized ileal digestibility of fat and fatty acids in growing pigs.
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DOI：
10.1186/s40104-022-00798-w
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发表时间：
2022-12-14
影响因子：
7
通讯作者：
Lai, Changhua
中科院分区：
文献类型：
--
作者：
Wang, Lu;Gao, Wenjun;Zhou, Junyan;Shi, Huangwei;Wang, Tenghao;Lai, Changhua
在国家饲料数据库中缺乏脂肪和脂肪酸的标准化回肠消化率（SID）数据。此外，重要的是要指定用于脂肪分析的程序。因此，进行了一项试验，以1）测定生长猪在10种不同油源中脂肪和脂肪酸的表观回肠消化率（AID）和SID，并开发基于脂肪酸组成的脂肪SID预测方程;和2）比较脂肪提取方法对脂肪内源损失和消化率计算值的影响。将22头公猪（初始体重：32.1 ± 2.3 kg）在回肠远端手术安装T形插管，并在4阶段Youden Square设计中分配到11种实验饲料中的1种。用玉米淀粉、大豆分离蛋白和蔗糖配制无脂饲料。10个添加油的饲料，通过添加6%的膳食油源，以玉米淀粉为代价的无脂饲料配制。所有日粮均含有26%甜菜渣和0.40%氧化铬。乙醚提取物（EE）的内源性损失低于酸解脂肪（AEE; P < 0.01）。脂肪和饱和脂肪酸的AID和SID在不同油源间差异显著（P < 0.05）。棕榈油AEE的SID低于向日葵籽油、玉米油、菜籽油、稻米油和亚麻籽油（P < 0.01）。脂肪的AID和SID分别为79.65%~ 86.97%和91.14%~ 99.18%。尽管EE的AID大于AEE（P < 0.01），但除棕榈油外，EE和AEE的SID无显著差异。不饱和脂肪酸与饱和脂肪酸的比值（U/S）与脂肪SID呈正相关（P < 0.05），C16：0和长链饱和脂肪酸（LSFA）与脂肪SID呈显著负相关（P < 0.01）。预测脂肪SID的最佳拟合方程为SID AEE = 102.75 − 0.15 × LSFA − 0.74 × C18：0 − 0.03 × C18：1（调整决定系数= 0.88，P < 0.01）。在计算脂肪的SID时，样品的EE含量可采用直接提取法进行分析，而脂肪的AID应采用酸水解法进行测定。日粮脂肪消化率受脂肪酸组成的影响，尤其是C16：0、LSFA和U/S含量的影响。在线版本包含补充材料，可通过10.1186/s40104-022-00798-w获得。
There is a lack of data for the standardized ileal digestibility (SID) of fat and fatty acids in national feed databases. In addition, it is important to specify the procedures used for fat analyses. Therefore, an experiment was conducted to 1) determine the apparent ileal digestibility (AID) and SID of fat and fatty acids in ten different oil sources for growing pigs and to develop prediction equations for SID of fat based on fatty acid composition; and 2) compare the effect of the fat extraction methods on the calculated values for endogenous loss and digestibility of fat. Twenty-two barrows (initial body weight: 32.1 ± 2.3 kg) were surgically fitted with a T-cannula in the distal ileum, and allotted to 1 of 11 experimental diets in a 4-period Youden Square design. A fat-free diet was formulated using cornstarch, soy protein isolate and sucrose. Ten oil-added diets were formulated by adding 6% of dietary oil sources to the fat-free diet at the expense of cornstarch. All diets contained 26% sugar beet pulp and 0.40% chromic oxide. The endogenous loss of ether extract (EE) was lower than that of acid-hydrolyzed fat (AEE; P < 0.01). There were significant differences in the AID and SID of fat and saturated fatty acids across the dietary oil sources (P < 0.05). The SID of AEE for palm oil was lower than that of sunflower oil, corn oil, canola oil, rice oil and flaxseed oil (P < 0.01). The AID and SID of fat ranged from 79.65% to 86.97% and from 91.14% to 99.18%. Although the AID of EE was greater than that of AEE (P < 0.01), there was no significant difference in SID of EE and AEE except for palm oil. The ratio of unsaturated to saturated fatty acids (U/S) had a positive correlation with SID of fat (P < 0.05), whereas C16:0 and long chain saturated fatty acids (LSFA) were significant negatively correlated with SID of fat (P < 0.01). The best-fit equation to predict SID of fat was SID AEE = 102.75 − 0.15 × LSFA − 0.74 × C18:0 − 0.03 × C18:1 (Adjusted coefficient of determination = 0.88, P < 0.01). When calculating the SID of fat, the EE content of the samples can be analyzed using the direct extraction method, whereas the acid hydrolysis procedure should be used to determine the AID of fat. Fat digestibility of dietary oils was affected by their fatty acid composition, especially by the contents of C16:0, LSFA and U/S. The online version contains supplementary material available at 10.1186/s40104-022-00798-w.
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影响因子：
3.2
作者：
Gu, X;Li, D
通讯作者：
Li, D
影响因子：
3.3
作者：
Kellner, T. A.;Patience, J. F.
通讯作者：
Patience, J. F.
影响因子：
3.3
作者：
LLOYD, LE;CRAMPTON, EW;MACKAY, VG
通讯作者：
MACKAY, VG
影响因子：
3.3
作者：
Mendoza, S. M.;van Heugten, E.
通讯作者：
van Heugten, E.
影响因子：
1.8
作者：
Lauridsen, Charlotte;Hedemann, Mette Skou;Jensen, Soren Krogh
通讯作者：
Jensen, Soren Krogh