Live Imaging of Axonal Dynamics After Laser Axotomy of Peripheral Neurons in Zebrafish.
Live Imaging of Axonal Dynamics After Laser Axotomy of Peripheral Neurons in Zebrafish.
复制标题
斑马鱼周围神经元激光轴突切除术后轴突动力学的实时成像。
DOI：
10.1007/978-1-0716-3012-9_14
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Sagasti,Alvaro
中科院分区：
文献类型：
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作者：
Adula,KadidiaP;Sagasti,Alvaro
轴突切断导致不同的结果，包括成功再生和功能重建、再生失败或神经元细胞死亡。实验性损伤轴突使得研究从细胞体分离的远端残端的退化和记录再生的连续步骤成为可能。精确的损伤减少了对轴突周围环境的损伤，从而减少了外部过程的参与，如瘢痕形成或炎症，使研究人员能够分离出内在因素在再生中发挥的作用。有几种方法被用来切断轴突，每种方法都有其优点和缺点。本章介绍了使用双光子显微镜上的激光切割斑马鱼幼虫的触觉神经元的单个轴突，并实时共聚焦成像来监测其再生，这种方法提供了卓越的分辨率。
Axon severing results in diverse outcomes, including successful regeneration and reestablishment of function, failure to regenerate, or neuronal cell death. Experimentally injuring an axon makes it possible to study degeneration of the distal stump that was detached from the cell body and document the successive steps of regeneration. Precise injury reduces damage to the environment surrounding an axon, and thereby the involvement of extrinsic processes, such as scarring or inflammation, enabling researchers to isolate the role that intrinsic factors play in regeneration. Several methods have been used to sever axons, each with advantages and disadvantages. This chapter describes using a laser on a two-photon microscope to cut individual axons of touch-sensing neurons in zebrafish larvae, and live confocal imaging to monitor its regeneration, a method that provides exceptional resolution.