Characterization of Marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells.
Characterization of Marek's disease virus-infected lymphocytes: discrimination between cytolytically and latently infected cells.
复制标题
DOI：
--
复制
发表时间：
1983-03
期刊：
影响因子：
--
通讯作者：
W. Shek;Calnek Bw;Schat Ka;Chen-lo H. Chen-Chen-lo-H.-Chen-2109482134
中科院分区：
文献类型：
--
作者：
W. Shek;Calnek Bw;Schat Ka;Chen-lo H. Chen-Chen-lo-H.-Chen-2109482134
对遗传性马立克病（MD）有抗性的n系鸡和对MD敏感的p系鸡在暴露于MD病毒的JM-10克隆后的不同时间分离白细胞悬液。在暴露后3和5天（DPE），在生产限制（细胞溶解）阶段，大多数感染的脾脏和胸腺白细胞被发现是低密度的尼龙毛粘附细胞，具有Fc受体和表面Ia和IgM，并被羰基铁处理耗尽。来自n系和p系鸡的白细胞都是如此。相比之下，大多数来自潜伏期（即7 DPE后）的p系鸡的感染脾细胞没有发现上述特征，只有一个例外：潜伏感染细胞的表面显示有Ia抗原。这些实验表明，JM-10细胞溶解感染的主要目标是b细胞，而随后的潜伏感染主要在非b淋巴细胞中发现。
Leukocyte suspensions derived from genetically Marek's disease (MD)-resistant N-line and MD-susceptible P-line chickens were fractionated at various times after exposure to the JM-10 clone of MD virus. At 3 and 5 days post exposure (DPE), during the productive-restrictive (cytolytic) phase, most infected spleen and thymus leukocytes were found to be low-density, nylon wool-adherent cells that possessed Fc receptors and surface Ia and IgM and were depleted by carbonyl iron treatment. This was true for leukocytes derived from N-line as well as those from P-line chickens. In contrast, most infected spleen cells derived from P-line chickens during the latent phase (i.e., after 7 DPE) were not found to have the above characteristics, with one exception: Ia antigen was demonstrated on the surface of latently infected cells. From these experiments it was concluded that the principal targets of the cytolytic JM-10 infection are B-cells, whereas the subsequent latent infection was found mostly in non-B-lymphocytes.