Model plants for studying the interaction between Methylobacterium mesophilicum and Xylella fastidiosa
Model plants for studying the interaction between Methylobacterium mesophilicum and Xylella fastidiosa
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DOI：
10.1139/w05-142
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发表时间：
2006-05-01
影响因子：
2.8
通讯作者：
Azevedo, Joao Lucio
中科院分区：
文献类型：
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作者：
Andreote, Fernando D.;Lacava, Paulo T.;Azevedo, Joao Lucio
在过去的几年中，与柑橘相关的内生细菌群落已被研究为与苛养木杆菌相互作用的关键组分。本研究以柑橘内生菌Methylobacterium mesophilicum SR1.6/6为研究对象，研究了其与柑橘X.在模式植物如长春花（Catharanthus roseus）（长春花长春花）和烟草（Nicotiana clevelandii）（荷兰烟草）中，本研究的目的是确定M. mesophilicum SR1.6/6接种后，roseus和N.采用PCR-DGGE（聚合酶链反应-变性梯度凝胶电泳）和平板接种技术，使用针对α-和β-变形菌门的特异性引物，在接种菌株SR1.6/6的植物中观察到土著内生细菌群落的变化。在接种M. mesophilicum SR1.6/6.这强调了这些组织作为该生物体的主要入口点的重要性。结果表明：C. roseus和N. clevelandii可作为研究M. mesophilicum和X.苛养。
Over the last few years, endophytic bacterial communities associated with citrus have been studied as key components interacting with Xylella fastidiosa. In this study, we investigated the possible interaction between the citrus endophyte Methylobacterium mesophilicum SR1.6/6 and X. fastidiosa in model plants such as Catharanthus roseus (Madagaskar periwinkle) and Nicotiana clevelandii (Clevelands tobacco). The aim of this study was to establish the fate of M. mesophilicum SR1.6/6 after inoculation of C. roseus and N. clevelandii plants, using PCR-DGGE (polymerase chain reaction - denaturing gradient gel electrophoresis) and plating techniques. Shifts in the indigenous endophytic bacterial communities were observed in plants inoculated with strain SR1.6/6, using specific primers targeting alpha- and beta-Proteobacteria. Cells of strain SR1.6/6 were observed in a biofilm structure on the root and hypocotyl surfaces of in vitro seedlings inoculated with M. mesophilicum SR1.6/6. This emphasizes the importance of these tissues as main points of entrance for this organism. The results showed that C. roseus and N. clevelandii could be used as model plants to study the interaction between M. mesophilicum and X. fastidiosa.