Optically controllable muscle for cell-based microdevice
Optically controllable muscle for cell-based microdevice
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DOI：
10.1109/mhs.2014.7006150
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发表时间：
2014-11
期刊：
影响因子：
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通讯作者：
T. Asano;T. Ishizuka;H. Yawo;K. Morishima
中科院分区：
文献类型：
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作者：
T. Asano;T. Ishizuka;H. Yawo;K. Morishima
结合了组织、细胞和生物分子等生物成分的生物微器件引起了人们对新型工程器件的极大关注。特别是由生化能量反应驱动的肌肉驱动的微执行器，还可以节省能源、资源和空间。凭借这些优势，传统上，收缩肌肉已被应用于使用电场刺激的工程化微型设备。电场刺激是一种控制收缩活动时间模式的简单方法。然而，它通常是不均匀的，许多意想不到的肌肉细胞同时受到刺激。为了提高空间和时间分辨率，我们从表达光门离子通道的小鼠C2C12成肌细胞中制备了光敏骨骼肌细胞，通道视紫红质(ChR)。光脉冲使表达Chr的肌肉的膜电位去极化，并最终诱发出动作电位。它还以一种与给定模式的LED脉冲同时发生的方式诱导抽搐样收缩。这项技术将在生物工程领域有许多应用，例如肌肉动力执行器/微设备的无线驱动。
Biomicrodevices incorporating biological components such as tissues, cells and biomolecules have raised much attention for novel engineering devices. Particularly, the muscle-powered microactuator driven by biochemical energy reaction would also save energy, resource and space. With these advantages, conventionally, contractile muscles have been applied to engineered microdevices using electrical field stimulation. Electrical field stimulation is a simple method to control the temporal pattern of contractile activity. However, it is generally nonuniform and many unexpected muscle cells are stimulated simultaneously. To improve both the spatial and temporal resolutions, we made photosensitive skeletal muscle cells from murine C2C12 myoblasts, which express light-gated ion channel, channelrhodopsin (ChR). The light pulse depolarized the membrane potential of a ChR-expressing muscle and eventually evoked an action potential. It also induced a twitch-like contraction in a concurrent manner with a given pattern of LED pulses. This technique would have many applications in the bioengineering field, such as wireless drive of muscle powered actuators/microdevices.