T-lymphocyte maturation abnormalities in uninfected newborns and children with vertical exposure to HIV
T-lymphocyte maturation abnormalities in uninfected newborns and children with vertical exposure to HIV
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DOI：
10.1182/blood.v96.12.3866.h8003866_3866_3871
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发表时间：
2000-12-01
期刊：
影响因子：
20.3
通讯作者：
Vigano, A
中科院分区：
文献类型：
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作者：
Clerici, M;Saresella, M;Vigano, A
感染 HIV 的儿童的细胞介导的免疫和 T 淋巴细胞成熟受到损害。如果 HIV 或其可溶性蛋白能够穿过胎盘屏障，则可以在 HIV 女性未感染 HIV 的后代（血清恢复者 [SR]）中检测到这些异常。对 HIV 感染母亲 (SR) 的 20 名健康未感染 HIV 的新生儿和 HIV 阴性妇女 (UC) 的 14 名健康新生儿进行了免疫表型分析。在 3 组年龄较大的儿童中进行了相同的分析：SR (n = 41)； UC（n = 15）；和 HIV 感染儿童 (n = 25)，使用 ELISpot 和荧光分析评估抗原特异性细胞；采用酶联免疫吸附法（ELISA）测定血清IL-7浓度，结果显示SR新生儿：（1）CD4/CD8比值降低，（2）CD4（+）和CD8（+）幼稚T细胞百分比降低，（3）活化CD8（+）T细胞百分比增加，（4）CD3（+）/4（-）/8（-）（DN）百分比和DN/25(-)/44(+) 有所增强。这些异常在老年 SR 儿童中部分保留，在部分新生儿 SR 中检测到 CD4(+) 和 CD8(+) HIV 特异性细胞，但在老年 SR 中未检测到，新生儿和老年 SR 中血清 IL-7 均升高，即使在感染 HIV 的母亲的未感染 HIV 的新生儿中，细胞介导的免疫和 T 细胞成熟也发生改变；这些异常现象会随着时间的推移而持续存在。这些观察结果的生物学意义和潜在的后续临床事件应该在更大的血清逆转者队列中进行研究。 (C) 2000 年，美国血液学会。
Cell-mediated immunity and T-lymphocyte maturation are impaired in HIV-infected children. These abnormalities would be detected in HIV-uninfected offspring of HIV women (seroreverters [SR]) if HIV or its soluble proteins could cross the placental barrier. Immunophenotypic analyses were performed in 20 healthy HIV-uninfected newborns of HIV-infected mothers (SR), and in 14 healthy newborns of HIV-negative women (UC), The same analyses were performed in 3 groups of older children: SR (n = 41); UC (n = 15); and HIV-infected children (n = 25), Anti-gen-specific cells were evaluated with ELISpot and fluorimetric analyses; IL-7 serum concentration was measured by enzyme-linked immunosorbent assay (ELISA), Results showed that in SR newborns: (1) the CD4/CD8 ratio was reduced, (2) CD4(+) and CD8(+) naive T-cell percentages were decreased,(3)percentage of activated CD8(+) T cells was increased, and (4) percentages of CD3(+)/4(-)/8(-) (DN) and DN/25(-)/44(+) were augmented. These abnormalities were partially retained in older SR children, CD4(+) and CD8(+) HIV-specific cells were detected in a portion of newborn SRs but not in older SRs, Serum IL-7 was augmented both in newborn and older SRs, Cell-mediated immunity and T-cell maturation are altered even in HIV-uninfected newborns of HIV-infected mothers; these abnormalities persist over time. The biologic significance of these observations and potential subsequent clinical events should be investigated in larger cohorts of seroreverters. (C) 2000 by The American Society of Hematology.