Experimental model for the irradiation-mediated abscopal effect and factors influencing this effect.
Experimental model for the irradiation-mediated abscopal effect and factors influencing this effect.
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DOI：
10.14989/doctor.k22826
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发表时间：
2020-02
影响因子：
5.3
通讯作者：
Kiichiro Baba;M. Nomura;S. Ohashi;T. Hiratsuka;Y. Nakai;Tomoki Saito;Y. Kondo;K. Fukuyama;Osamu Kikuchi;Atsushi Yamada;J. Matsubara;Kenshiro Hirohashi;Y. Mitani;A. Mizumoto;M. Muto
中科院分区：
文献类型：
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作者：
Kiichiro Baba;M. Nomura;S. Ohashi;T. Hiratsuka;Y. Nakai;Tomoki Saito;Y. Kondo;K. Fukuyama;Osamu Kikuchi;Atsushi Yamada;J. Matsubara;Kenshiro Hirohashi;Y. Mitani;A. Mizumoto;M. Muto
放射治疗（RT）是癌症的主要治疗方法。来自放射治疗的电离辐射在照射部位诱发肿瘤损伤，尽管在临床上并不常见，但可能导致远离照射部位的肿瘤消退，这种现象被称为离体效应。近年来，抽离效应与癌症患者总生存时间的延长有关，但影响抽离效应的因素尚不清楚。本研究的目的是阐明影响超视距效应的因素。在此，我们建立了一个小鼠模型，我们诱导了抽离效应。我们将MC38（小鼠结肠腺癌）细胞皮下注射到C57BL/6小鼠的两个部位。只有一个肿瘤被照射，两个肿瘤的大小随时间被测量。非照射部位肿瘤消退，显示出体外作用。这种效果通过增加照射肿瘤体积和抗pd1抗体的施用而增强。放射治疗联合抗pd1抗体诱导的体外效应还受放射剂量和照射肿瘤体积的影响。这些现象在其他细胞系B16F10细胞（小鼠黑色素瘤细胞）中也得到了证实。这些发现提供了进一步的证据的机制和影响因素，体外效应在RT。
Radiotherapy (RT) is the primary treatment for cancer. Ionizing radiation from RT induces tumor damage at the irradiated site, and, although clinically infrequent, may cause regression of tumors distant from the irradiated site-a phenomenon known as the abscopal effect. Recently, the abscopal effect has been related to prolongation of overall survival time in cancer patients, though the factors that influence the abscopal effect are not well understood. The aim of this study is to clarify the factors influencing on abscopal effect. Here, we established a mouse model in which we induced the abscopal effect. We injected MC38 (mouse colon adenocarcinoma) cells subcutaneously into C57BL/6 mice at two sites. Only one tumor was irradiated and the sizes of both tumors were measured over time. The non-irradiated-site tumor showed regression, demonstrating the abscopal effect. This effect was enhanced by an increase in the irradiated-tumor volume and by administration of anti-PD1 antibody. When the abscopal effect was induced by a combination of RT and anti-PD1 antibody, it was also influenced by radiation dose and irradiated-tumor volume. These phenomena were also verified in other cell line, B16F10 cells (mouse melanoma cells). These findings provide further evidence of the mechanism for, and factors that influence, the abscopal effect in RT.