Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum.
Peptide YY, Glucagon-Like Peptide-1, and Neurotensin Responses to Luminal Factors in the Isolated Vascularly Perfused Rat Ileum.
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DOI：
10.1210/endo.139.9.6202
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发表时间：
1998-09
期刊：
影响因子：
4.8
通讯作者：
V. Dumoulin;F. Moro;A. Barcelo;T. Dakka;J. Cuber
中科院分区：
文献类型：
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作者：
V. Dumoulin;F. Moro;A. Barcelo;T. Dakka;J. Cuber
回肠暴露于营养物质明显抑制几种上消化道功能。回肠壁的激素肽，即肽YY（PYY）、胰高血糖素样肽-1（GLP-1）和神经降压素（NT），被认为在这种负反馈机制中起作用。本研究进行比较评估分泌PYY，GLP-1，和NT后，各种单独的管腔因子在离体血管灌注大鼠回肠制备的管腔输注。PYY，GLP-1，和NT测定门静脉流出物与特定RIA。葡萄糖（250 mM）诱导三种肽的显著释放，而5 mM的生理浓度不诱导肽分泌。蛋白胨（5%，wt/vol）诱发PYY、GLP-1和NT的持续释放。只有NT分泌增加后，管腔管理的100 mM油酸钠。短链脂肪酸（20 mM）引起的三种肽的早期和短暂的释放。相反，牛磺胆酸盐（20 mM）诱导PYY、GLP-1和NT的持续释放，但NT的肽释放阈值浓度低于PYY或GLP-1。纤维素或果胶（0.5%，重量/体积）没有改变肽分泌。总之，葡萄糖和蛋白胨是PYY、GLP-1和NT释放的有效刺激剂。只有NT在油酸刺激下释放。最后，牛磺胆酸盐是三种肽释放的有效刺激剂。总之，PYY、GLP-1和NT可能协同参与回肠制动。由于引发肽释放需要相对高浓度的各种刺激物，因此似乎这种机制在消化不良或吸收不良的情况下起作用。
Exposure of the ileum to nutrients markedly inhibits several upper gastrointestinal functions. Hormonal peptides of the ileal wall, i.e. peptide YY (PYY), glucagon-like peptide-1 (GLP-1), and neurotensin (NT), are thought to play a role in this negative feedback mechanism. The present study was conducted to comparatively assess the secretion of PYY, GLP-1, and NT upon luminal infusion of a variety of individual luminal factors in the isolated vascularly perfused rat ileum preparation. PYY, GLP-1, and NT were measured in the portal effluent with specific RIAs. Glucose (250 mM) induced a pronounced release of the three peptides, whereas a physiological concentration of 5 mM did not induce peptide secretion. Peptone (5%, wt/vol) evoked a sustained release of PYY, GLP-1, and NT. Only NT secretion was increased upon luminal administration of 100 mM sodium oleate. Short chain fatty acids (20 mM) evoked an early and transient release of the three peptides. In contrast, taurocholate (20 mM) induced a sustained release of PYY, GLP-1, and NT, but the threshold concentration for peptide release was lower for NT than for PYY or GLP-1. Cellulose or pectin (0.5%, wt/vol) did not modify peptide secretion. In conclusion, glucose and peptone are potent stimulants of PYY, GLP-1, and NT release. Only NT is released upon oleic acid stimulation. Finally, taurocholate is a potent stimulant of the release of the three peptides. Overall, PYY, GLP-1, and NT may participate cooperatively in the ileal brake. As relatively high concentrations of the various stimulants were required to elicit peptide release, it seems likely that this mechanism operates in cases of maldigestion or malabsorption.