Mapping of QTLs associated with cadmium tolerance and accumulation during seedling stage in rice (Oryza sativa L.)
Mapping of QTLs associated with cadmium tolerance and accumulation during seedling stage in rice (Oryza sativa L.)
复制标题
水稻苗期与镉耐受性和积累相关的 QTL 定位（Oryza sativa L.）
DOI：
10.1007/s10681-008-9785-3
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发表时间：
2009-02-01
期刊：
影响因子：
1.9
通讯作者：
Zhang, Guoping
中科院分区：
文献类型：
--
作者：
Xue, Dawei;Chen, Mingcan;Zhang, Guoping
镉（Cd）是一种非必需元素，对植物有毒害作用。为研究水稻耐镉和镉积累的遗传规律，以粳稻JX 17和籼稻ZYQ 8杂交的双单倍体（DH）群体为材料，定位了水稻苗期耐镉和镉积累的数量性状基因座（QTL）。共检测到22个QTL，其中对照和Cd胁迫下分别检测到10个和12个QTL，这些QTL与株高（SH）、根长（RL）、地上部干重（SDW）、根干重（RDW）、总干重（TDW）和叶绿素含量（CC）相关。在耐镉系数（CTC）上检测到6个QTL，分别位于第1、3、5、8和10染色体上。在Cd胁迫下，3个控制根系和地上部Cd含量的QTL分别定位在第6和第7染色体上。在第3染色体上发现了一个镉浓度的地上/地下比率QTL。结果表明，水稻对Cd的耐性和积累量具有数量遗传性，所检测到的QTL可用于标记辅助选择（MAS）和鉴定控制水稻对Cd耐性和积累量的基因。
Cadmium (Cd) is a non-essential element and toxic to plants. To investigate the genetics of Cd tolerance and accumulation in rice, quantitative trait loci (QTL) associated with Cd tolerance and accumulation at the seedling stage were mapped using a doubled haploid (DH) population derived from a cross between a japonica JX17 and an indica ZYQ8. A total of 22 QTLs were found to be associated with shoot height (SH), root length (RL), shoot dry weight (SDW), root dry weight (RDW), total dry weight (TDW) and chlorophyll content (CC), and 10 and 12 QTLs were identified under the control and Cd stress conditions, respectively. For Cd tolerant coefficient (CTC), 6 QTLs were detected on chromosomes 1, 3, 5, 8 and 10. Under Cd stress, 3 QTLs controlling root and shoot Cd concentrations were mapped on chromosome 6 and 7. One QTL for shoot/root rate of Cd concentration was identified on chromosome 3. The results indicated that Cd tolerance and accumulation were quantitatively inherited, and the detected QTLs may be useful for marker-assistant selection (MAS) and identification of the genes controlling Cd tolerance and accumulation in rice.