IL-5 synthesis is upregulated in human nasal polyp tissue
IL-5 synthesis is upregulated in human nasal polyp tissue
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DOI：
10.1016/s0091-6749(97)80019-x
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发表时间：
1997-06-01
影响因子：
14.2
通讯作者：
Rudack, C
中科院分区：
文献类型：
--
作者：
Bachert, C;Wagenmann, M;Rudack, C
背景资料：在大多数鼻息肉中，组织嗜酸性粒细胞增多是一个引人注目的发现，其病理机制尚不清楚。目的：本研究进行了调查，可能不同的细胞因子和趋化因子的模式，可以解释的特征性组织嗜酸性粒细胞增多的鼻polypy.Methods：息肉从23例患者和18例对照组的鼻甲组织进行了调查。通过ELISA测量组织匀浆的细胞因子蛋白含量（IL-1 β、IL-3、IL-4、IL-5、IL-6、IL-8、IL-10、肿瘤坏死因子-α、粒细胞-巨噬细胞集落刺激因子、IL-IRA、RANTES、GRO-α）。结果：IL-5在18例正常鼻息肉组织中仅1例阳性，而在23例鼻息肉组织中有18例阳性。免疫组化显示大量IL-5阳性细胞，其中69.5%可通过形态学鉴定为嗜酸性粒细胞。在所有标本中均检测到IL-6、IL-8、IL-10、肿瘤坏死因子-α、GRO-α和RANTES，组间无显著差异（p>或等于0.05），而在鼻甲粘膜中发现IL-1 β和IL-1 RA浓度显著较高（p < 0.05）。未检测到IL-3;结论：IL-5在嗜酸性鼻息肉的病理生理过程中起重要作用，IL-5可能由嗜酸性粒细胞产生。
Background: In most nasal polyps, tissue eosinophilia is a striking finding, the pathologic mechanism of which is not understood.Objective: This study was performed to investigate a possibly distinct cytokine and chemokine pattern that could explain the characteristic tissue eosinophilia in nasal polyps.Methods: Polyps from 23 patients and turbinate tissue from 18 control subjects were investigated. The cytokine protein content (IL-1 beta, IL-3, IL-4, IL-5, IL-6, IL-8, IL-10, tumor necrosis factor-alpha, granulocyte-macrophage colony-stimulating factor, IL-IRA, RANTES, GRO-alpha) of tissue homogenates was measured by ELISA. Immunohistochemistry was performed in selected samples to detect IL-5(+), major basic protein-positive, and EG2(+) cells.Results: IL-5 was detectable in only one sample of tissue from 18 control subjects but was found in 18 of 23 nasal polyps. Immunohistochemistry revealed an abundant number of IL-5(+) cells, of which 69.5% could be identified as eosinophils by morphology. IL-6, IL-8, IL-10, tumor necrosis factor-alpha, GRO-alpha, and RANTES were detected in all specimens, without significant differences between groups (p greater than or equal to 0.05), whereas significantly higher concentrations of IL-1 beta and IL-1RA were found in turbinate mucosa (p < 0.05). IL-3 was not detectable; granulocyte-macrophage colony-stimulating factor could only occasionally be found.Conclusion: This study indicates that IL-5 plays a keg role in the pathophysiology of eosinophilic nasal polyps and may be produced by eosinophils.