A new En face method is useful to quantitate endothelial damage in vivo
A new En face method is useful to quantitate endothelial damage in vivo
复制标题
DOI：
10.1016/j.bbrc.2003.08.013
复制
发表时间：
2003-09-19
影响因子：
3.1
通讯作者：
Mitsumata, M
中科院分区：
文献类型：
--
作者：
Azuma, K;Watada, H;Mitsumata, M
内皮损伤被认为是动脉粥样硬化过程中的初始变化。然而，很难在体内检测到这种初始变化。我们建立了一种改良的En面免疫染色方法，使我们能够获得清晰的图像的整个内皮表面，包括在动脉分叉，并定量的细胞在内皮感兴趣的数量。使用这种方法，我们发现，治疗与致动脉粥样硬化因子，白蛋白衍生的晚期糖基化终产物，只有2周的增殖细胞核抗原阳性内皮细胞和粘附在内皮细胞的巨噬细胞的数量显着增加，这表明这些变化可能是相关的内皮功能障碍的早期事件。总之，本改良En面免疫染色方法可能是一个很有前途的工具，了解动脉粥样硬化的病理生理。(C)2003年爱思唯尔公司All rights reserved.
Endothelial damage is considered to be an initial change in the atherosclerotic process. However, it has been difficult to detect this initial change in vivo. We established a modified En face immunostaining method that enabled us to obtain clear images of the entire endothelial surface, including at arterial bifurcations, and to quantitate the number of cells of interest in the endothelium. Using this method, we found that treatment with an atherogenic factor, albumin-derived advanced glycosylation end products, for only 2 weeks caused a significant increase in the number of proliferating cell nuclear antigen-positive endothelial cells and the number of macrophages adhering to the endothelium, suggesting that these changes might be relevant to the early events of endothelial dysfunction. In conclusion, the present modified En face immunostaining method may be a promising tool for understanding the pathophysiology of atherosclerosis. (C) 2003 Elsevier Inc. All rights reserved.