Increased extracellular pressure provides a novel adjuvant stimulus for enhancement of conventional dendritic cell maturation strategies.
Increased extracellular pressure provides a novel adjuvant stimulus for enhancement of conventional dendritic cell maturation strategies.
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DOI：
10.1016/j.bbrc.2009.07.010
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发表时间：
2009-09-11
影响因子：
3.1
通讯作者：
Basson, Marc D.
中科院分区：
文献类型：
--
作者：
Craig, David H.;Shiratsuchi, Hiroe;Basson, Marc D.
近年来，以树突状细胞(DC)为基础的疫苗策略越来越受欢迎。体外产生具有免疫功能的成熟DC的方法仍需优化。树突状细胞已被证明在高压下表型成熟。我们比较了压力与内毒素刺激和细胞因子刺激对DC成熟的影响。将人单核细胞来源的未成熟DC或内毒素和细胞因子成熟的DC暴露于常压或40毫米汞加压12小时，然后检测CD80、CD86、CD40、MHC-I/II的表达和炎性细胞因子的产生。通过与同种异体淋巴细胞共培养，还评估了DC刺激T细胞增殖的能力。压力显著增加了未成熟DC上除MHC-I和CD40外的所有表面分子的表达和细胞因子的产生。压力/脂多糖处理的DC进一步上调MHC-I、CD40和IL-12p70。细胞因子成熟的DC对压力的反应似乎较差。T细胞增殖与MHC表达呈正相关。结果提示，机械刺激DC可能为TLR激动剂成熟策略提供有用的佐剂。
Dendritic cell (DC)-based vaccine strategies have gained increasing popularity in recent years. Methods for ex vivo generation of immunocompetent mature DCs still require optimization. DCs have been shown to phenotypically mature under elevated pressure. We compared the effects of pressure on DC maturation with LPS- and cytokine-stimulation. Human monocyte-derived immature or LPS- and cytokine-matured DCs were exposed to ambient or 40mmHg increased pressure for 12-hrs., then assessed for expression of CD80, CD86, CD40, MHC-I/II, and inflammatory cytokine production. DCs were also evaluated for capacity to stimulate T-cell proliferation by co-culture with allogeneic lymphocytes. Pressure significantly increased cytokine production and expression of all surface molecules on immature DC other than MHC-I and CD40. Pressure/LPS-treated DCs displayed further upregulation of MHC-I, CD40, and IL-12p70. Cytokine-matured DCs appeared less responsive to pressure. T-cell proliferation correlated with MHC expression. Results suggest mechanical stimulation of DCs may provide a useful adjuvant to TLR-agonist maturation strategies.
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影响因子：
56.9
作者：
Huang, Q;Liu, DY;Hacohen, N
通讯作者：
Hacohen, N
影响因子：
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作者：
Shiratsuchi, H;Basson, MD
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作者：
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作者：
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