Effects of culture conditions on glutathione content in A549 cells.
Effects of culture conditions on glutathione content in A549 cells.
复制标题
培养条件对 A549 细胞中谷胱甘肽含量的影响。
DOI：
10.1016/0006-291x(83)90842-2
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发表时间：
1983
影响因子：
3.1
通讯作者：
Menzel,DB
中科院分区：
文献类型：
--
作者：
Post,GB;Keller,DA;Connor,KA;Menzel,DB
研究了不同培养条件对A549（人ⅱ型肺肿瘤源）细胞谷胱甘肽含量的影响。研究的参数包括生长时间、血清浓度以及是否存在胰岛素、转铁蛋白和亚硒酸的混合物。谷胱甘肽含量随血清浓度升高而升高。当细胞与血清一起培养时，谷胱甘肽在传代后24小时急剧升高，随后下降。胰岛素、转铁蛋白和亚硒酸对细胞生长和谷胱甘肽含量影响不大。用含有10%血清的新鲜培养基替代培养液并不能阻止谷胱甘肽的生长依赖性下降。这些结果表明，A549细胞的谷胱甘肽含量受培养条件的强烈影响。
The effects of varying culture conditions on glutathione content in A549 (human type II lung tumor derived) cells were examined. Parameters studied were growth time, serum concentration, and the presence or absence of a mixture of insulin, transferrin, and selenous acid. Glutathione content increased with serum concentration. When cells were grown with serum, glutathione increased sharply 24 hours after passage and decreased thereafter. Insulin, transferrin, and selenous acid had little effect on cell growth or glutathione content. Replacement of media with fresh media containing 10% serum did not prevent the growth dependent decrease in glutathione. These results demonstrate that glutathione content in A549 cells is strongly affected by culture conditions.