Association analyses between genetic polymorphisms of endothelial nitric oxide synthase gene and hypertension in Japanese: The Suita Study
Association analyses between genetic polymorphisms of endothelial nitric oxide synthase gene and hypertension in Japanese: The Suita Study
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DOI：
10.1097/00004872-200111000-00003
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发表时间：
2001-11-01
影响因子：
4.9
通讯作者：
Iwai, N
中科院分区：
文献类型：
--
作者：
Tsujita, Y;Baba, S;Iwai, N
目的 内皮源性一氧化氮在血管张力的调节中发挥关键作用。最近，据报道内皮一氧化氮合酶（eNOS）基因多态性与高血压或冠状动脉痉挛有关。我们在 4055 名日本人的大型人群样本中研究了 eNOS 基因多态性与高血压之间的关联。 设计和方法 我们研究了 eNOS 基因的两种多态性：外显子 7 的 Glu298Asp 多态性和启动子区域的 T(-786)C 多态性。将高血压受试者的基因型分布与其他受试者的基因型分布进行比较。在未服用高血压药物的受试者中分析了基因型对血压值的影响。通过荧光素酶报告基因检测检测(-786)T或(-786)C等位基因的eNOS基因启动子活性。结果两个多态性之间存在显着的连锁不平衡(P < 0.0001)。 Glu298Asp 或 T(-786)C 多态性的基因型分布在高血压患者和其他受试者之间没有差异。未服用高血压药物的受试者的血压在Glu298Asp或T(-786)C多态性的三种基因型之间没有观察到显着差异。在用 (-786)T 和 (-786)C 等位基因转染的牛内皮细胞之间没有观察到启动子活性存在显着差异。结论我们的数据表明，eNOS 基因的这些多态性不太可能是所研究的日本人群中高血压易感性的主要因素。 (C) 2001 Lippincott Williams& Wilkins。
Objectives Endothelium-derived nitric oxide plays a key role in the regulation of vascular tone. Recently, endothelial nitric oxide synthase (eNOS) gene polymorphisms were reported to be associated with hypertension or coronary spasm. We investigated the association between the eNOS gene polymorphisms and hypertension in a large population-based sample of 4055 Japanese.Design and methods We investigated two polymorphisms of the eNOS gene, Glu298Asp polymorphism of exon 7 and T(-786)C polymorphism of the promoter region. The genotype distribution in hypertensive subjects was compared to that in the other subjects. The influence of the genotype on blood pressure values was analyzed in the subjects not taking hypertensive medication. The promoter activities of the eNOS gene with the (-786)T or (-786)C allele were measured by a luciferase reporter gene assay.Results There was significant linkage disequilibrium between the two polymorphisms (P < 0.0001). The genotype distribution of the Glu298Asp or T(-786)C polymorphism did not differ between the hypertensive and the other subjects. No significant differences in the blood pressure of subjects not taking hypertensive medication were observed among the three genotypes of Glu298Asp or T(-786)C polymorphisms. No significant differences in the promoter activity were observed between bovine endothelial cells transfected with the (-786)T and (-786)C alleles.Conclusions Our data suggested that these polymorphisms of the eNOS gene are unlikely to be major factors in the susceptibility to hypertension in the Japanese population studied. (C) 2001 Lippincott Williams& Wilkins.