Strains of Toxoplasma gondii used for tachyzoite antigens to stimulate spleen cells of infected mice in vitro affect cytokine responses of the cells in the culture.
Strains of Toxoplasma gondii used for tachyzoite antigens to stimulate spleen cells of infected mice in vitro affect cytokine responses of the cells in the culture.
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用于速殖子抗原的弓形虫菌株在体外刺激受感染小鼠的脾细胞，影响培养物中细胞的细胞因子反应。
DOI：
10.1007/s00436-005-1416-5
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发表时间：
2005
影响因子：
2
通讯作者：
Suzuki,Yasuhiro
中科院分区：
文献类型：
--
作者：
Rodgers,Laurel;Wang,Xisheng;Wen,Xiangshu;Dunford,Bradley;Miller,Renee;Suzuki,Yasuhiro
Cytokine responses of lymphocytes againstToxoplasma gondiiare usually studied by stimulating the cells from infected animals with tachyzoite lysate antigens (TLA) in vitro. We examined whether strains (genotypes) of the parasite used for TLA affect the production of IL-2, IL-4, IL-10 and IFN-γ in the culture of spleen cells obtained from mice infected with the ME49 (type II) strain. The amounts of IL-2 and IL-4 detected in the culture supernatants following stimulation with TLA of the RH (type I) strain were significantly greater than those detected following stimulation with TLA of the ME49 strain at 2 and 4 weeks after infection, respectively. These results suggest that antigen variations among the strains ofT. gondiiaffect cytokine production of lymphocytes of infected mice. Therefore, to obtain accurate information on cytokine production by immune lymphocytes of infected hosts, it is important to use the identical strain ofT. gondiifor both infection of animals and preparation of TLA to stimulate the lymphocytes in vitro.
