The cytochrome system of sea urchin eggs and embryos.
The cytochrome system of sea urchin eggs and embryos.
复制标题
海胆卵和胚胎的细胞色素系统。
DOI：
10.1016/0003-9861(83)90030-9
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发表时间：
1983
影响因子：
3.9
通讯作者：
E. Nakano
中科院分区：
文献类型：
--
作者：
K. Okabayashi;E. Nakano
对马粪海胆卵和胚胎细胞色素系统进行了研究。线粒体组分的差谱表明，在未受精卵中存在完整的细胞色素系统。测定了受精前后卵粗提物中细胞色素水平和呼吸酶活性。未受精卵含有细胞色素a3、B和c + c1，比例为1.0：1.8：0.7。原肠胚含有几乎相同数量的细胞色素a3和bas未受精卵。而原肠胚中cytochromec+ c1的含量是未受精卵的1.5倍。细胞色素氧化酶活性在发育过程中保持不变。未受精卵中未发现细胞色素氧化酶抑制剂。抗霉素A敏感和不敏感的NADH-细胞色素还原酶活性在发育过程中增加。琥珀酸-细胞色素还原酶活性在发育早期上升，达到一个暂时的平台，然后下降，在pluteus阶段。这些结果进行了讨论，在早期发展过程中呼吸的增加。
The cytochrome system in eggs and embryos of the sea urchin,Hemicentrotus pulcherrimus, was investigated. Difference spectra of the mitochondrial fraction demonstrated the presence of a complete cytochrome system in unfertilized eggs. Cytochrome levels and the activities of respiratory enzymes were measured in crude extracts of eggs both before and after fertilization. Unfertilized eggs contained cytochromesaa3,b, andc+c1in a ratio of 1.0:1.8:0.7. Gastrulae contained almost the same amount of cytochromesaa3andbas unfertilized eggs. However, the amount of cytochromec+c1in gastrulae was 1.5 times greater than that in unfertilized eggs. The activity of cytochrome oxidase remained unchanged during development. No cytochrome oxidase inhibitor was found in unfertilized eggs. Both antimycin A-sensitive and insensitive NADH-cytochromecreductase activities increased during development. The activity of succinate-cytochromecreductase increased during early development, reached a temporary plateau, and then declined at the pluteus stage. These results are discussed in relation to the increase of respiration during early development.