Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI
Feasibility of noninvasive evaluation of biophysical properties of tissue-engineered cartilage by using quantitative MRI
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DOI：
10.1016/j.jbiomech.2007.02.002
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发表时间：
2007-01-01
影响因子：
2.4
通讯作者：
Ushida, Takashi
中科院分区：
文献类型：
--
作者：
Miyata, Shogo;Numano, Tomokazu;Ushida, Takashi
组织工程化软骨在临床环境中的应用需要一种非侵入性方法来评估工程化软骨的生物物理和生化特性。由于关节软骨由70-80%的水组成，并且具有致密的细胞外基质（ECM），因此认为组织中的水分子的状况与其生物力学性质相关。因此，磁共振成像（MRI）是一种潜在的方法来评估工程软骨的生物物理特性。在这项研究中，我们测试的假设，定量MRI可以作为一种非侵入性的评估方法，以评估工程软骨的生物物理特性。为了重建软骨组织模型，将从小牛肱骨头收获的软骨细胞包埋在琼脂糖凝胶中并在体外培养长达4周。平衡杨氏模量由应力松弛试验确定。在机械测试之后，测量MRI导出的参数（纵向弛豫时间T1、横向弛豫时间T2和水自扩散系数D）。工程软骨的平衡杨氏模量随培养时间的增加而增加，而T1和D则下降。基于回归分析，T1和D显示出与平衡杨氏模量的强相关性。结果表明，MRI测量的T1和D值可用于无创监测工程软骨的生物物理特性。(c)2007爱思唯尔有限公司保留所有权利。
The application of tissue-engineered cartilage in a clinical setting requires a nonirivasive method to assess the biophysical and biochemical properties of the engineered cartilage. Since articular cartilage is composed of 70-80% water and has dense extracellular matrixes (ECM), it is considered that the condition of the water molecules in the tissue is correlated with its biomechanical property. Therefore, magnetic resonance imaging (MRI) represents a potential approach to assess the biophysical property of the engineered cartilage. In this study, we test the hypothesis that quantitative MRI can be used as a noninvasive assessment method to assess the biophysical property of the engineered cartilage. To reconstruct a model of cartilaginous tissue, chondrocytes harvested from the humeral head of calves were embedded in an agarose gel and cultured in vitro up to 4 weeks. Equilibrium Young's moduli were determined from the stress relaxation tests. After mechanical testing, MRI-derived parameters (longitudinal relaxation time T1, transverse relaxation time 72, and water self-diffusion coefficient D) were measured. The equilibrium Young's modulus of the engineered cartilage showed a tendency to increase with an increase in the culture time, whereas T1 and D decreased. Based on a regression analysis, T1 and D showed a strong correlation with the equilibrium Young's modulus. The results showed that T1 and D values derived from the MRI measurements could be used to noninvasively monitor the biophysical properties of the engineered cartilage. (c) 2007 Elsevier Ltd. All rights reserved.