Removal of Cells from Body Fluids by Magnetic Separation in Batch and Continuous Mode: Influence of Bead Size, Concentration, and Contact Time
Removal of Cells from Body Fluids by Magnetic Separation in Batch and Continuous Mode: Influence of Bead Size, Concentration, and Contact Time
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DOI：
10.1021/acsami.7b10140
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发表时间：
2017-09-07
影响因子：
9.5
通讯作者：
Herrmann, Inge K.
中科院分区：
文献类型：
--
作者：
Bohmer, Nils;Demarmels, Nino;Herrmann, Inge K.
从体液中磁性分离致病化合物是一个吸引人的治疗概念。最近，已经证明使用体外透析型装置可以去除多种病原体。在这种装置中，流体和磁珠之间的接触时间被限制在几分钟内。这带来了挑战，特别是如果需要去除细菌或细胞等大型化合物。在这里，我们报告的可行性，以消除细胞从体液中的连续透析类型的设置。我们使用一系列不同的磁珠尺寸（50-4000 nm）、浓度和接触时间，评估了从含有或不含白色血细胞的生理液体中去除肿瘤细胞的效率。我们表明，肿瘤细胞可以在可接受的时间内（1-2分钟）和珠浓度（0.2 mg/mL）从体液中定量去除。我们进一步提出了一个数学模型来描述在竞争性非特异性摄取的存在下，去除一定数量的细胞所需的最小珠数浓度。目前的研究铺平了道路的调查研究，以评估磁血液净化细胞去除的治疗潜力，在类似透析的设置。
The magnetic separation of pathogenic compounds from body fluids is an appealing therapeutic concept. Recently, removal of a diverse array of pathogens has been demonstrated using extracorporeal dialysis-type devices. The contact time between the fluid and the magnetic beads in such devices is limited to a few minutes. This poses challenges, particularly if large compounds such as bacteria or cells need to be removed. Here, we report on the feasibility to remove cells from body fluids in a continuous dialysis type of setting. We assessed tumor cell removal efficiencies from physiological fluids with or without white blood cells using a range of different magnetic bead sizes (50-4000 nm), concentrations, and contact times. We show that tumor cells can be quantitatively removed from body fluids within acceptable times (1-2 min) and bead concentrations (0.2 mg per mL). We further present a mathematical model to describe the minimal bead number concentration needed to remove a certain number of cells, in the presence of competing nonspecific uptake. The present study paves the way for investigational studies to assess the therapeutic potential of cell removal by magnetic blood purification in a dialysis-like setting.