INHIBITION OF NUCLEIC ACID-BINDING PROTEINS BY AURINTRICARBOXYLIC ACID
INHIBITION OF NUCLEIC ACID-BINDING PROTEINS BY AURINTRICARBOXYLIC ACID
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DOI：
10.1016/0006-291x(73)91198-4
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发表时间：
1973-01-01
影响因子：
3.1
通讯作者：
LANDERS, TA
中科院分区：
文献类型：
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作者：
BLUMENTHAL, T;LANDERS, TA
Qβ复制酶、大肠杆菌RNA聚合酶和T7 RNA聚合酶可被低浓度的染料金精三羧酸（ATA）抑制。在每种情况下，酶的引发优先受到抑制。在抑制起始所需的浓度范围内，Qβ复制酶引发的多核苷酸链的延伸对ATA不敏感。用ATA处理Qβ复制酶、RNA聚合酶和lac阻遏物可阻止模板与硝酸纤维素滤膜的酶介导结合。我们建议抑制剂与这些蛋白质的模板结合位点结合，以防止启动。
Qβ replicase, Escherichia coli RNA polymerase, and T7 RNA polymerase are inhibited by low concentrations of the dye aurintricarboxylic acid (ATA). In each case initiation by the enzyme was preferentially inhibited. The elongation of initiated polynucleotide chains by Qβ replicase was insensitive to ATA in the range of concentrations required to inhibit initiation. Treatment of Qβ replicase, RNA polymerase and lac repressor with ATA prevented enzymemediated binding of the templates to nitrocellulose filters. We propose that the inhibitor combines with the template binding site of these proteins to prevent initiation.