Variation of Synechococcus Pigment Genetic Diversity Along Two Turbidity Gradients in the China Seas
Variation of Synechococcus Pigment Genetic Diversity Along Two Turbidity Gradients in the China Seas
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DOI：
10.1007/s00248-017-1021-z
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发表时间：
2017-07
影响因子：
3.6
通讯作者：
X. Xia;Hongbin Liu;D. Choi;J. Noh
中科院分区：
文献类型：
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作者：
X. Xia;Hongbin Liu;D. Choi;J. Noh
集球藻是一种重要且广泛分布的微蓝细菌，具有丰富的色素多样性。本研究以聚球藻基因组DNA为模板，设计引物（peBF/peAR），扩增聚球藻基因组DNA中的peBA操纵子序列，研究中国近海聚球藻色素在沿着两个浊度梯度上的多样性。我们的数据显示，所有以前报道的色素类型发生在南（SCS）和东（ECS）中国海。此外，还发现了一种新的色素遗传类型（3f型），以高藻胆素聚球藻为代表。检出KORDI-100菌株（Exc 495：545 = 2.35）。这种色素遗传类型与3c/3d型的不同之处不仅在于PUB/PEB比值很高，而且在于藻胆体的基因间隔区序列和基因组织结构不同。夏季南海沿岸沃茨以2型为主，大洋沃茨以3c/3d和3f型为主。然而，在ECS中，3a型是整个样带的主要色素类型。因此，在海洋环境中，多种色素类型共存，但以一种色素类型为主，PUB/PEB比值与聚球藻色素类型的地理分布有关。中国两个边缘海集球藻色素组成有明显差异。
Synechococcusare important and widely distributed picocyanobacteria that encompass a high pigment diversity. In this study, we developed a primer set (peBF/peAR) for amplifying thecpeBAoperon sequence fromSynechococcusgenomic DNA to studySynechococcuspigment diversity along two turbidity gradients in the China seas. Our data revealed that all previously reported pigment types occurred in the South (SCS) and East (ECS) China Seas. In addition, a novel pigment genetic type (type 3f), represented by the high phycourobilinSynechococcussp. strain KORDI-100 (Exc495:545 = 2.35), was detected. This pigment genetic type differs from the 3c/3d types not only for a very high PUB/PEB ratio but also for a different intergenic spacer sequence and gene organization of the phycobilisome.Synechococcusof different pigment types exhibited clear niche differentiation. Type 2 dominated in the coastal waters, whereas type 3c/3d and 3f were predominant in oceanic waters of the SCS in summer. In the ECS, however, type 3a was the major pigment type throughout the transect. We suggest that in marine environment, various pigment types often co-occur but with one type dominant and PUB/PEB ratio is related to geographic distribution ofSynechococcuspigment types. The two marginal seas of China have markedly differentSynechococcuspigment compositions.