Exogenous synovial stem cells adhere to defect of meniscus and differentiate into cartilage cells.
Exogenous synovial stem cells adhere to defect of meniscus and differentiate into cartilage cells.
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DOI：
10.11480/jmds.550112
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发表时间：
2008-03
影响因子：
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通讯作者：
K. Mizuno;T. Muneta;Toshiyuki Morito;S. Ichinose;H. Koga;A. Nimura;T. Mochizuki;I. Sekiya
中科院分区：
文献类型：
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作者：
K. Mizuno;T. Muneta;Toshiyuki Morito;S. Ichinose;H. Koga;A. Nimura;T. Mochizuki;I. Sekiya
半月板是半月纤维软骨，其损伤导致膝关节功能障碍。我们先前报道了滑膜间充质干细胞（MSCs）的高软骨形成潜力。在这里，我们研究了关节内注射的滑膜间充质干细胞是否粘附在半月板的缺损处，在那里存活，并分化成软骨细胞。从GFP大鼠的滑膜中分离MSC。在野生大鼠的膝关节中制造圆柱形缺损，并将GFP阳性的滑膜MSC注射到膝关节中。在对照组中，将100微升PBS注射到对侧膝关节中。第1天、第2、4、8和12周后分析半月板。在注射10（7）个GFP阳性滑膜MSC后一天，滑膜缺损被细胞填充。GFP阳性的滑膜间充质干细胞表达II型胶原，在8周时通过电子显微镜观察显示出软骨细胞的代表性特征，并且在12周时仍然可以观察到。组织学评分在12周内有所改善，但在该模型中，两组之间在每个时期均无统计学差异。在第1天，在远端器官中未观察到GFP mRNA表达。关节腔内注射后，滑膜间充质干细胞附着于关节缺损处并分化为软骨细胞。
The meniscus is semilunar fibrocartilage, and its injury causes dysfunction of the knee. We previously reported a high chondrogenic potential of synovial mesenchymal stem cells (MSCs). Here, we examined whether intra-articular injected synovial MSCs adhered to the defect of the meniscus, survived there, and differentiated into cartilage cells. MSCs were isolated from the synovium of GFP rats. Cylindrical defects were created in the menisci in wild rats, and GFP-positive synovial MSCs were injected into the knee. In the control group, 100 microl of PBS was injected into the contralateral knee. The menisci were analyzed after day 1, weeks 2, 4, 8, and 12. One day after injecting of 10(7) GFP-positive synovial MSCs, the meniscal defect was filled with the cells. The GFP-positive synovial MSCs expressed type II collagen, exhibited representative characteristics of chondrocytes by electron microscopy at 8 weeks, and could still be observed at 12 weeks. The histological score improved within 12 weeks but there were no statistical difference between the two groups at each period in this model. GFP mRNA expressions were not observed in distant organs at day 1. After intra-articular injection, synovial MSCs attached to the meniscal defect and differentiated into cartilage cells.