Arachidonic acid increases intracellular calcium in erythrocytes.
Arachidonic acid increases intracellular calcium in erythrocytes.
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DOI：
10.1016/s0006-291x(02)00327-3
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发表时间：
2002-05
影响因子：
3.1
通讯作者：
L. Soldati;C. Lombardi;D. Adamo;A. Terranegra;C. Bianchin;G. Bianchi;G. Vezzoli
中科院分区：
文献类型：
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作者：
L. Soldati;C. Lombardi;D. Adamo;A. Terranegra;C. Bianchin;G. Bianchi;G. Vezzoli
最近，我们在红细胞中测量了电压调节和二氢吡啶抑制的钙流入。由于花生四烯酸和其他多不饱和脂肪酸影响大多数离子通道的活性，我们研究了它们对红细胞 Ca2+ 内流的影响。使用荧光染料 Fura 2 作为 [Ca2+]i 指示剂，对从健康供体分离的新鲜红细胞进行测量。 AA (5–50μM) 和 EPA (20–30μM) 刺激源自细胞外钙 (1mM) 的 [Ca2+]i 浓度依赖性增加，而不影响细胞内和细胞外 pH 值和膜电压。 AA 存在时，Ca2+ 流入速率为 0.5 至 3nM Ca2+/s，EPA 存在时，Ca2+ 流入速率为 0.9 至 1.7nM Ca2+/s。 AA 和 EPA 引起的 Ca2+ 内流不受二氢吡啶的抑制，而环氧合酶抑制剂有效，PGE1 或 PGE2 没有产生任何作用。我们得出结论，AA可以通过环氧合酶途径的中间产物激活红细胞电压独立的Ca2+转运；然而，不能排除与膜脂蛋白的直接相互作用。
Recently, we have measured in erythrocytes a voltage-modulated and dihydropyridine-inhibited calcium influx. Since arachidonic acid and other polyunsaturated fatty acids influence the activities of most ion channels, we studied their effects on the erythrocyte Ca2+influx. It was measured on fresh erythrocytes, isolated from healthy donors, using the fluorescent dye Fura 2 as indicator of [Ca2+]i. AA (5–50μM) and EPA (20–30μM) stimulated a concentration-dependent increase in [Ca2+]i, deriving from extracellular calcium (1mM), without affecting the intra- and extracellular pH and membrane voltage. The Ca2+influx rate varied from 0.5 to 3nM Ca2+/s in the presence of AA and from 0.9 to 1.7nM Ca2+/s with EPA. The Ca2+influx elicited by AA and EPA was not inhibited by dihydropyridines, while cyclooxygenase inhibitors were effective and PGE1 or PGE2 did not produce any effect. We conclude that AA could activate an erythrocyte voltage-independent Ca2+transport via an intermediate product of cyclooxygenase pathway; however, a direct interaction with the membrane lipid–protein cannot be excluded.