Development of micro-injection as an innovative autologous fat graft technique: The use of adipose tissue as dermal filler
Development of micro-injection as an innovative autologous fat graft technique: The use of adipose tissue as dermal filler
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DOI：
10.1016/j.bjps.2012.06.014
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发表时间：
2012-12-01
影响因子：
2.7
通讯作者：
Magalon, G.
中科院分区：
文献类型：
--
作者：
Nguyen, P. S. A.;Desouches, C.;Magalon, G.
背景资料：自体脂肪移植，由于其体积质量和对皮肤营养的作用，可以被认为是金标准植入物。目前的技术不允许非常浅表或皮下注射脂肪组织。作者报告了通过25号套管转移脂肪的技术改进。移植的脂肪的活力进行评估后，皮下注射在小鼠model.Methods：微脂肪移植包括收获脂肪组织使用多孔套管与孔的1毫米。脂肪组织细化所描述的SR科尔曼和转移通过微套管。最初，人类脂肪首先使用两种不同的程序收获：科尔曼技术和改进的收获技术。进行了初步的比较组织学分析。16只裸鼠接受人脂肪组织：一侧使用科尔曼技术通过17-G插管填充0.7 cc，另一侧使用较小的插管（20 G、23 G和25 G）按照改良的收获技术移植。结果：对小鼠实验性脂肪移植物进行了观察和分析：大体上，每侧的脂肪组织显示出相同的健康外观。苏木精-伊红-伊红染色显示完整的脂肪细胞，抗CD 31抗体免疫组化染色显示丰富的新生血管网络。结论：改良技术获得的脂肪移植物保持了正常的组织学结构。使用微套管的脂肪注射将lipostructure的应用扩展到皮肤的表层。(c)2012年英国整形、重建和美容外科医生协会。由爱思唯尔有限公司出版。保留所有权利。
Background: Autologous fat graft, by virtue of its volumetric qualities and its action on skin trophicity, can be considered as a gold standard implant. Current techniques do not allow very superficial or subdermal injections of adipose tissue. The authors report technical modifications that enable fat transfer through a 25-gauge cannula. The viability of grafted fat was assessed after subcutaneous injection on a murine model.Methods: Micro-fat grafting consists of harvesting fat tissue using a multiperforated cannula with holes of 1 mm. Fat tissue is refined as described by SR Coleman and transferred through a micro-cannula. Initially, human fat was first harvested using two different procedures: Coleman's technique and the modified harvesting technique. Preliminary comparative histologic analyses were performed. Sixteen nude mice received human fat tissue: one side was filled with 0.7 cc through a 17-G cannula using Coleman's technique, and the opposite side was grafted using smaller cannulae (20G, 23G and 25G) following the modified harvesting technique. Mice were euthanised at 12 weeks and skin biopsies were performed.Results: Experimental fat grafts on mice were observed and analysed: macroscopically, the fat tissue of each side showed the same healthy aspect. Haematoxylin-eosin-saffron staining revealed intact adipocytes and anti-CD31 antibody immunohistochemical staining highlighted an abundant neo-vessel network.Conclusion: Fat graft obtained by the modified technique maintains a normal histologic structure. Fat injection with micro-cannulae extends the application of lipostructure to the superficial layers of the skin. (c) 2012 British Association of Plastic, Reconstructive and Aesthetic Surgeons. Published by Elsevier Ltd. All rights reserved.