Arginase release from red blood cells: Possible link in transfusion induced immune suppression?
Arginase release from red blood cells: Possible link in transfusion induced immune suppression?
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DOI：
10.1097/00024382-200116020-00005
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发表时间：
2001-08-01
期刊：
影响因子：
3.1
通讯作者：
van Leeuwen, PAM
中科院分区：
文献类型：
--
作者：
Prins, HA;Houdijk, APJ;van Leeuwen, PAM
精氨酸刺激淋巴细胞功能，并被红细胞中大量存在的一种酶-精氨酸酶降解。精氨酸酶在体外损害淋巴细胞功能和反应。同种异体输血中储存的红细胞中还原酶的泄漏可能与淋巴细胞功能障碍有关。在本研究中，在储存用于输血的红细胞袋中测定了凝血酶活性。常规地从4名健康供体获得血沉棕黄层耗尽的红细胞，并在4 ℃下在袋中储存最长5周。对袋进行取样，以测定乳酸脱氢酶、乳酸脱氢酶和钾。此外，随机抽取了36袋即将输注给患者的红细胞样本进行研究。精氨酸酶、乳酸脱氢酶和钾的水平在四个供体的袋中显示出时间依赖性增加。精氨酸酶活性的这种时间依赖性增加可以在额外取样的袋中得到证实（P < 0.0001，r = 0.78）。结果首次表明，在输血储存过程中，红细胞中会释放出还原酶。精氨酸酶输注可能在输血后观察到的免疫抑制中起重要作用。
Arginine stimulates lymphocyte function and is degraded by arginase, an enzyme that is abundantly present in red blood cells. Arginase impairs lymphocyte function and responses in vitro. Leakage of arginase from stored red blood cells may be involved in the lymphocyte dysfunction associated in allogenic blood transfusion. In the present study, arginase activity was determined in bags of red cells stored for transfusion. Buffy coat depleted red blood cells were obtained routinely from four healthy donors and stored In bags for a maximum period of five weeks at 4 degreesC. The bags were sampled for determination of arginase, lactate dehydrogenase, and potassium. In addition, a random sample of 36 bags of red blood cells about to be transfused to patients were studied. Levels of arg[nase, lactate dehydrogenase, and potassium showed a time dependent increase in the bags of the four donors. This time dependent increase in arg[nase activity could be confirmed in the additional bags sampled (P < 0.0001, r = 0.78). The results for the first time show that arginase is released from red blood cells during storage for transfusion. Arginase infusion may play an important role in the Immune suppression observed after blood transfusion.