Investigating mitochondrial radical production using targeted probes
Investigating mitochondrial radical production using targeted probes
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DOI：
10.1042/bst0321011
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发表时间：
2004-12-01
影响因子：
3.9
通讯作者：
Murphy, MP
中科院分区：
文献类型：
--
作者：
Murphy, MP
已经开发了一系列靶向线粒体的探针分子，其包含共价连接到活性部分的亲脂性阳离子。在线粒体膜电位的驱动下，亲脂性阳离子导致这些分子积累到线粒体中。迄今为止，我们已经将抗氧化剂、自旋陷阱、硫醇试剂和 DNA 烷基化化合物靶向线粒体。这些化合物的选择性线粒体定位使我们能够研究线粒体产生活性氧的几个方面。
A range of mitochondria-targeted probe molecules that comprise a lipophilic cation covalently attached to an active moiety have been developed. The lipophilic cation causes the accumulation of these molecules into mitochondria, driven by the mitochondrial membrane potential. To date, we have targeted antioxidants, spin traps, thiol reagents and DNA-alkylating compounds to mitochondria. The selective mitochondrial localization of these compounds enables us to investigate several aspects of the production of reactive oxygen species by mitochondria.