Characterizing the molecular and morphophysiological diversity of Italian red clover
Characterizing the molecular and morphophysiological diversity of Italian red clover
复制标题
表征意大利红三叶草的分子和形态生理多样性
作者：
M. Pagnotta;P. Annicchiarico;A. Farina;S. Proietti
三叶草（Trifolium pratense L.）是欧洲的第三大牧草物种，但在该地区进化的地方品种和自然种群的生物多样性和遗传结构的信息有限。本研究的目的是产生这样的信息，意大利种质的分子和形态生理多样性的基础上。这项研究包括来自广泛环境的16个意大利自然种群，代表四种传统商业生态型的四个地方品种和两个品种。8个形态生理性状进行了评估，在一个重复的试验在田间条件下，而两个AFLP引物组合与140个多态性标记被记录在一个随机样本的13个基因型每个人口。排序和分类结果的基础上的形态生理学数据清楚地跟踪种质的类型（即地方品种或自然种群）及其地理起源，不像基于分子标记的结果。根据Mantel检验，基于形态生理性状的群体间欧氏距离与基于分子标记的Nei遗传距离不相关。地方品种或自然群体材料之间的地理距离与基于形态生理性状的距离相关，但与基于分子标记的距离无关。通过分子标记估计的平均群体内的变化是约2.6倍，高于群体间，防止大多数群体之间的可接受的歧视。平均而言，自然群体往往有类似品种的群体内变异，略低于地方品种。我们的研究结果对红三叶草遗传资源的收集、保护、开发和登记具有重要意义。
Red clover (Trifolium pratense L.) is the third major forage species in Europe, but there is limited information on the biodiversity and the genetic structure of landraces and natural populations which evolved in this region. The objective of this study was producing such information for Italian germplasm on the ground of molecular and morphophysiological diversity. The study included 16 Italian natural populations from a wide range of environments, four landraces representing the four traditional commercial ecotypes, and two varieties. Eight morphophysiological traits were assessed in a replicated trial under field conditions, whereas two AFLPs primer combinations with 140 polymorphic markers were recorded on a random sample of 13 genotypes per population. Ordination and classification results based on morphophysiological data clearly kept track of the type of germplasm (i.e. landrace or natural population) and its geographic origin, unlike results based on molecular markers. Euclidean distances among populations based on morphophysiological traits were not correlated with Nei’s genetic distances based on molecular markers according to Mantel’s test. Geographical distances among landrace or natural population material was correlated with distances based on morphophysiological traits but not with those based on molecular markers. The average within-population variation estimated via molecular markers was about 2.6-fold higher than that among populations, preventing an acceptable discrimination among most populations. On average, natural populations tended to have within-population variation similar to varieties and somewhat lower than landraces. Our results have implications for collection, conservation, exploitation and registration in a sui generis system of red clover genetic resources.
影响因子：
3.3
作者：
L. Excoffier;P. Smouse;J. Quattro
通讯作者：
L. Excoffier;P. Smouse;J. Quattro