PM2.5 exposure perturbs lung microbiome and its metabolic profile in mice
PM2.5 exposure perturbs lung microbiome and its metabolic profile in mice
复制标题
PM2.5暴露扰乱小鼠肺部微生物组及其代谢特征
DOI：
10.1016/j.scitotenv.2020.137432
复制
发表时间：
2020-06-15
影响因子：
9.8
通讯作者：
Chen, Chengshui
中科院分区：
文献类型：
--
作者：
Li, Jingli;Hu, Yiran;Chen, Chengshui
细颗粒物（PM2.5）由于其对健康的不利影响，已成为一个主要的公共卫生问题。肺部是受PM2.5影响的主要器官。为了解PM2. 5致肺损伤的机制，采用16 S rRNA基因测序和液相色谱-质谱（LC-MS）代谢组学分析方法，研究PM2. 5暴露对肺微生物组及其代谢特征的影响。小鼠肺内滴注PM2.5，建立肺损伤模型。16 S rRNA基因测序表明，PM2.5暴露显著改变了肺部微生物组的丰富度，均匀度和组成。代谢组学分析表明，肺代谢物的水平受到扰动后，PM2.5暴露。代谢物的改变主要属于代谢途径，如柠檬酸循环、乙醛酸和二羧酸代谢、丙酮酸代谢、嘌呤和嘧啶代谢以及缬氨酸、亮氨酸和异亮氨酸代谢。改变的肺微生物群显示出与肺代谢物的显著相关性。富马酸水平与瘤胃球菌科、肠杆菌科和假单胞菌科的相对丰度呈负相关。这些结果表明，PM2.5暴露不仅显著改变了肺部微生物组的组成，而且还干扰了参与不同代谢途径的许多代谢物。本研究加深了我们对PM2.5暴露后肺损伤机制的理解。(C)2020年，任作家。由爱思唯尔公司出版
Fine particulate matter (PM2.5) have become a major public health concern because of their adverse effects on health. Lungs are considered the primary organ affected by PM2.5. In order to understand the mechanismunderlying PM2.5-induced lung injury, 16S rRNA gene sequencing, and liquid chromatography-mass spectrometry (LC-MS) metabolomics analysis were conducted to investigate the impact of PM2.5 exposure on lung microbiome and its metabolic profile. Mice were exposed to PM2.5 through intratracheal instillation and a lung injury model was established. 16S rRNA gene sequencing indicated that PM2.5 exposure significantly altered the richness, evenness, and composition of the lung microbiome. Metabolomics profiling showed that the levels of lung metabolites were perturbed after PM2.5 exposure. The altered metabolites mainly belonged to metabolic pathways, such as the citrate cycle, glyoxylate and dicarboxylate metabolism, pyruvate metabolism, purine and pyrimidine metabolism, and valine, leucine, and isoleucine metabolism. The altered lung microbiota showed significant correlations with lung metabolites. The levels of fumaric acid negatively correlated with the relative abundance of Ruminococcaceae, Enterobacteriaceae, and Pseudomonadaceae. These results revealed that PM2.5 exposure not only significantly altered the lung microbiome composition but also perturbed a number of metabolites involved in diverse metabolic pathways. This study improves our understanding of the mechanism of lung injury after PM2.5 exposure. (C) 2020 The Authors. Published by Elsevier B.V.