Corrigendum to "A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters" [Water Res. 126, 101-110].
Corrigendum to "A highly specific Escherichia coli qPCR and its comparison with existing methods for environmental waters" [Water Res. 126, 101-110].
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“高度特异性的大肠杆菌 qPCR 及其与环境水域现有方法的比较”的勘误表 [Water Res。
DOI：
10.1016/j.watres.2019.06.070
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发表时间：
2019
期刊：
影响因子：
12.8
通讯作者：
Walker DI
中科院分区：
文献类型：
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作者：
Walker DI
我们注意到方法第2.6节“实时PCR”中存在错误。发表的文章指出“每个反应含有12.5 μl IQ SYBR Supermix（含反应缓冲液、dNTP、Taq聚合酶和SYBR绿色II DNA结合染料（Biorad））、9.5 μl RT-PCR级水（Agilent）、1 ml各引物（终浓度5 mM）和1 ml模板DNA（100 ng/μl）;终体积为25 μl。"然而，这应该是“每个反应含有12.5 μl IQ SYBR Supermix（含反应缓冲液、dNTP、Taq聚合酶和SYBR绿色II DNA结合染料（Biorad））、9.5 μl RT-PCR级水（Agilent）、1 μl各引物（终浓度0.4 μM）和1 μl模板DNA（100 ng/μl）;最终体积为25 μl”。
It has been brought to our attention that there is an error in the Methods, section 2.6 “Real-time PCR”. The article as published states “Each reaction contained 12.5 μl of IQ SYBR Supermix con-taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 ml of each primer (final concentration 5 mM) and 1 ml of template DNA at 100 ng/μl; the final volume was 25 μl.”.However this should be “Each reaction contained 12.5 μl of IQ SYBR Supermix con-taining reaction buffer, dNTPs, Taq polymerase and SYBR Green II DNA binding dye (Biorad), 9.5 μl of RT-PCR grade water (Agilent), 1 μl of each primer (final concentration 0.4 μM) and 1 μl of template DNA at 100 ng/μl; the final volume was 25 μl.”