Bioassays for quantitating ciprofloxacin and tobramycin in aqueous humor.
Bioassays for quantitating ciprofloxacin and tobramycin in aqueous humor.
复制标题
定量房水中环丙沙星和妥布霉素的生物测定。
DOI：
10.1089/jop.1993.9.311
复制
发表时间：
1993
期刊：
影响因子：
--
通讯作者：
O'Callaghan,RJ
中科院分区：
文献类型：
--
作者：
Engel,LS;Callegan,MC;Hill,JM;O'Callaghan,RJ
细菌性角膜炎的新疗法的开发需要在受感染的眼组织中测定抗生素;然而，用于这种药物定量的方法可能是昂贵的，并且可能缺乏精确度和灵敏度。为了开发一种更合适的方法来定量房水中的妥布霉素和环丙沙星，比较了16种生物测定法。评估的变量包括：用作指示剂的细菌菌株（金黄色葡萄球菌ATCC 25923与肺炎克雷伯氏菌ATCC 10031）、细菌在琼脂表面的应用与在熔融琼脂中的内含物、琼脂的体积以及抗生素在纸片上的应用与向琼脂威尔斯孔中的滴注。当选择合适的指示生物（S. aureus对妥布霉素和K.肺炎球菌）施加到琼脂表面，将抗生素溶液放入琼脂威尔斯孔中，琼脂体积为30 ml/板。妥布霉素和环丙沙星的最佳检测灵敏度分别为0.98和0.12 μg/ml。当抗生素在眼房水或生理盐水中稀释时，这些敏感性提高了四倍。回归系数r > 0.90，精密度高。这些测定的灵敏度和精密度表明它们用于定量房水中的氟喹诺酮类和氨基糖苷类。
Development of new therapies for bacterial keratitis requires assays of antibiotics in infected ocular tissues; however, methods for such drug quantitation can be expensive and can lack precision and sensitivity. To develop a more suitable assay for quantitating tobramycin and ciprofloxacin in the aqueous humor, sixteen bioassays were compared. Variables assessed included: the strain of bacteria used as indicator (Staphylococcus aureusATCC 25923 versusKlebsiella pneumoniaeATCC 10031), the application of bacteria to the agar surface versus inclusion in molten agar, the volume of the agar, and the application of antibiotic on paper discs versus instillation into agar wells. The best results were obtained when the appropriate indicator organism (S. aureusfor tobramycin andK. pneumoniaefor ciprofloxacin) was applied to the surface of the agar, antibiotic solution was put into agar wells, and the volume of agar was 30 ml per plate. The best sensitivity for assays of tobramycin and ciprofloxacin diluted in water were 0.98 and 0.12 μg/ml, respectively. These sensitivities improved as much as fourfold when antibiotics were diluted in aqueous humor or normal saline. The precision of all assays tested was high as determined by regression coefficients (r > 0.90). The sensitivity and precision of these assays indicate their utility for quantitating fluoroquinolones and aminoglycosides in aqueous humor.