No need for biopsies: comparison of three sample techniques for wound microbiota determination
No need for biopsies: comparison of three sample techniques for wound microbiota determination
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DOI：
10.1111/j.1742-481x.2011.00883.x
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发表时间：
2012-06-01
影响因子：
3.1
通讯作者：
Krogfelt, Karen A.
中科院分区：
文献类型：
--
作者：
Gjodsbol, Kristine;Skindersoe, Mette E.;Krogfelt, Karen A.
该研究的目的是比较常规诊断中使用的三种采样技术，以识别慢性下肢静脉溃疡中的微生物群。共有46例持续性下肢静脉溃疡患者入选本研究。入选时，采集拭子、活检和滤纸垫样本。4周后，收集额外的活检和滤纸垫样本。通过标准方法在物种水平上分离和鉴定细菌。在89%的溃疡中发现的最常见的细菌种类是金黄色葡萄球菌。无耐甲氧西林S.我们没有发现三种采样技术之间分离的细菌种类有任何显著差异。然而，使用多种技术导致识别更多物种。我们的研究表明，使用拭子样本足以识别慢性伤口中存在的细菌种类，从而避免活检采样期间和之后的并发症。
The aim of the study was to compare three sampling techniques used in routine diagnostics to identify the microbiota in chronic venous leg ulcers. A total of 46 patients with persisting venous leg ulcers were included in the study. At inclusion, swab, biopsy and filter paper pad samples were collected. After 4 weeks, additional biopsy and filter paper pad samples were collected. Bacteria were isolated and identified at species level by standard methods. The most common bacterial species detected was Staphylococcus aureus found in 89% of the ulcers. No methicillin-resistant S. aureus isolates were found. We did not find any significant differences regarding the bacterial species isolated between the three sampling techniques. However, using multiple techniques led to identification of more species. Our study suggests that it is sufficient to use swab specimens to identify the bacterial species present in chronic wounds, thus avoiding complications during and after biopsy sampling.