Impact of subacute ruminal acidosis (SARA) adaptation and recovery on the density and diversity of bacteria in the rumen of dairy cows
Impact of subacute ruminal acidosis (SARA) adaptation and recovery on the density and diversity of bacteria in the rumen of dairy cows
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DOI：
10.1111/j.1574-6941.2011.01154.x
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发表时间：
2011-11-01
影响因子：
4.2
通讯作者：
McBride, Brian W.
中科院分区：
文献类型：
--
作者：
Hook, Sarah E.;Steele, Michael A.;McBride, Brian W.
亚急性瘤胃酸中毒（SARA）是以瘤胃pH值下降和微生物干扰为特征的疾病。研究了高谷物饲喂后SARA适应和恢复对瘤胃细菌密度和多样性的影响。四个瘤胃插管奶牛饲喂干草饮食，过渡到65%的谷物饮食3周，并返回到干草饮食3周。在第0周（干草）、第1周和第3周（高谷物）以及第4周和第6周（干草）对瘤胃液、瘤胃固体和粪便进行取样。在第1周诊断为SARA，pH值低于5.6持续4.6 ± 1.4小时。高籽粒组瘤胃内细菌密度显著低于低籽粒组（P = 0.047）。在98%水平上评估了第0、3和6周的瘤胃液克隆文库，并解析了154个操作分类单位。第3周多样性与第0周差异显著，群落结构与第0周和第6周差异显著（P <0.0001）。属于厚壁菌门的克隆占主导地位。与干草日粮相比，高谷物日粮含有来自反刍月形单胞菌和反刍琥珀酸单胞菌的克隆，但缺乏真杆菌属。SARA适应被发现显着改变细菌密度，多样性和群落结构，进一步研究细菌在SARA适应中的作用。
Subacute ruminal acidosis (SARA) is characterized by ruminal pH depression and microbial perturbation. The impact of SARA adaptation and recovery on rumen bacterial density and diversity was investigated following high-grain feeding. Four ruminally cannulated dairy cows were fed a hay diet, transitioned to a 65% grain diet for 3 weeks, and returned to the hay diet for 3 weeks. Rumen fluid, rumen solids, and feces were sampled during weeks 0 (hay), 1 and 3 (high grain), and 4 and 6 (hay). SARA was diagnosed during week 1, with a pH below 5.6 for 4.6 +/- 1.4 h. Bacterial density was significantly lower in the rumen solids with high grain (P = 0.047). Rumen fluid clone libraries from weeks 0, 3, and 6 were assessed at the 98% level and 154 operational taxonomic units were resolved. Week 3 diversity significantly differed from week 0, and community structure differed from weeks 0 and 6 (P < 0.0001). Clones belonging to the phylum Firmicutes predominated. Compared with the hay diet, the high-grain diet contained clones from Selenomonas ruminantium and Succiniclasticum ruminis, but lacked Eubacterium spp. SARA adaptation was found to significantly alter bacterial density, diversity, and community structure, warranting further investigation into the role bacteria play in SARA adaptation.