Palladium mediated deallylation in fully aqueous conditions for native chemical ligation at aspartic and glutamic acid sites
Palladium mediated deallylation in fully aqueous conditions for native chemical ligation at aspartic and glutamic acid sites
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DOI：
10.1039/c8ob00890f
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发表时间：
2018-06-14
影响因子：
3.2
通讯作者：
Brik, Ashraf
中科院分区：
文献类型：
--
作者：
Jbara, Muhammad;Eid, Emad;Brik, Ashraf
报道了一种在Asp和Glu位点处应用酰肼前体作为肽硫酯并在Asp和Glu的侧链处进行烯丙基保护的有效的天然化学连接方法。在连接步骤之后，在完全水性条件下，使用水溶性钯络合物[Pd（烯丙基）Cl]（2）和谷胱甘肽在几分钟内有效地去除烯丙基保护。
An efficient native chemical ligation approach at Asp and Glu sites is reported applying a hydrazide precursor, as a peptide thioester, and allyl protection at the side chain of Asp and Glu. The allyl protection was efficiently removed, after the ligation step, using the water-soluble palladium complex [Pd(allyl)Cl](2) and glutathione within a few minutes under fully aqueous conditions.