Lactobacillus plantarum surface-displayed influenza antigens (NP-M2) with FliC flagellin stimulate generally protective immune responses against H9N2 influenza subtypes in chickens
Lactobacillus plantarum surface-displayed influenza antigens (NP-M2) with FliC flagellin stimulate generally protective immune responses against H9N2 influenza subtypes in chickens
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DOI：
10.1016/j.vetmic.2020.108834
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发表时间：
2020-10-01
影响因子：
3.3
通讯作者：
Wang, Chun-Feng
中科院分区：
文献类型：
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作者：
Li, Qiong-Yan;Xu, Man-Man;Wang, Chun-Feng
H9N2型禽流感病毒每年给家禽业造成严重的经济损失。诱导粘膜免疫反应的疫苗可能成功地对抗流感病毒感染，因为它的传播主要发生在粘膜中。为了开发新型高效的植物乳杆菌口服疫苗以控制禽流感病毒在禽业的传播，本研究构建并表达了流感病毒抗原NP和M2与植物乳杆菌表面的鼠伤寒沙门氏菌鞭毛蛋白Flc的融合。对雏鸡进行口服免疫，检测血清抗体、粘膜抗体和特异性细胞免疫。对禽流感病毒免疫雏鸡的效果进行了评价。结果表明，口服重组植物乳杆菌的雏鸡，除分泌型免疫球蛋白A(SIgA)水平较高外，还有较高水平的免疫球蛋白抗体(Ig G)。此外，在黏膜注射NC8-pSIP409pgsA‘-NP-M2-flic的情况下，融合可显著提高NP和M2特异性T细胞免疫水平。重组NC8-pSIP409-pGSA‘-NP-M2-FLIC可有效保护鸡抵抗流感病毒感染，降低肺内病毒滴度。我们的研究结果表明，植物乳杆菌表达流感病毒NP-M2和一种粘膜佐剂(Flc)，可能会为未来的动物提供一种用于防御AIV的黏膜疫苗候选。
The H9N2 avian influenza virus (AIV) causes serious economic losses to the poultry industry every year. Vaccines that induce a mucosal immune response may be successful against influenza virus infection because its transmission occurs primarily in the mucosa. To develop novel and potent oral vaccines based on Lactobacillus plantarum (L. plantarum) to control the spread of AIV in poultry industry, in the present study, we constructed and expressed fusions of the influenza antigens NP and M2 with the Salmonella Typhimurium flagellinprotein FliC on the surface of L. plantarum. Oral immunization of chicks was performed, and serum antibodies, mucosal antibodies, and specific cellular immunity were detected. Immunizing chicks with avian influenza virus was evaluated. The results showed high levels of IgG in addition to high levels of secretory immunoglobulin A (sIgA) in chickens orally administered recombinant L. plantarum. In addition, the fusion may significantly increase the levels of NP- and M2-specific T cell-mediated immunity in the case of mucosal administration of NC8-pSIP409pgsA'-NP-M2-FliC. Recombinant NC8-pSIP409-pgsA'-NP-M2-FliC mediated effectively protected chickens against influenza virus and reduced virus titers in the lung. Our study outcomes indicate that the expression of influenza NP-M2 and a mucosal adjuvant (FliC), by L. plantarum could generate a mucosal vaccine candidate for animals in the future to defend against AIVs.