Phenethyl isothiocyanate stimulates glucose uptake through the Akt pathway in C2C12 myotubes
Phenethyl isothiocyanate stimulates glucose uptake through the Akt pathway in C2C12 myotubes
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DOI：
10.1080/09168451.2019.1594675
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发表时间：
2019-03-29
影响因子：
1.6
通讯作者：
Nagasawa, Takashi
中科院分区：
文献类型：
--
作者：
Chiba, Maiko;Ito, Yoshiaki;Nagasawa, Takashi
异硫氰酸苯乙酯 (PEITC) 是一种存在于十字花科蔬菜中的芳香族异硫氰酸酯。多项研究表明，异硫氰酸盐调节各种细胞内信号通路，从而表现出抗炎和解毒活性。然而，人们对 PEITC 对葡萄糖代谢的影响知之甚少。在这项研究中，我们检查了 PEITC 是否促进小鼠骨骼肌细胞（C2C12 肌管）中的葡萄糖利用。 PEITC 诱导 C2C12 细胞中葡萄糖摄取、葡萄糖转运蛋白 4 (Glut4) 易位至质膜以及 Akt 和 ERK 激活。抑制 Akt 会抑制 PEITC 诱导的 Glut4 易位和葡萄糖摄取，而抑制 ERK 则不会。此外，PEITC 增加了 ErbB2 和 ErbB3 的磷酸化。使用泛 ErbB 抑制剂治疗可减少 Akt 激活以及 PEITC 诱导的后续葡萄糖摄取。这些结果表明 PEITC 通过 C2C12 肌管中的 ErbB/Akt 途径促进葡萄糖利用。因此，PEITC 可以作为一种膳食成分，对碳水化合物代谢产生有益影响。
Phenethyl isothiocyanate (PEITC) is an aromatic isothiocyanate present in cruciferous vegetables. Several studies have shown that isothiocyanates regulate various intracellular signaling pathways, and thereby show anti-inflammatory and detoxifying activities. However, little is known about the effects of PEITC on glucose metabolism. In this study, we examined whether PEITC promotes glucose utilization in mouse skeletal muscle cells, C2C12 myotubes. PEITC induced glucose uptake, glucose transporter 4 (Glut4) translocation to the plasma membrane, and activation of Akt and ERK in C2C12 cells. Inhibition of Akt suppressed PEITC-induced Glut4 translocation and glucose uptake, whereas ERK inhibition did not. Furthermore, PEITC increased phosphorylation of ErbB2 and ErbB3. Treatment with a pan-ErbB inhibitor reduced Akt activation and the subsequent glucose uptake induced by PEITC. These results indicate that PEITC promotes glucose utilization through the ErbB/Akt pathway in C2C12 myotubes. PEITC may therefore serve as a dietary constituent with beneficial effects on the carbohydrate metabolism.