Relationship Between Human β-Defensin 2 and the Vaginal Environment
Relationship Between Human β-Defensin 2 and the Vaginal Environment
复制标题
人β-防御素2与阴道环境的关系
DOI：
10.7883/yoken.jjid.2019.190
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发表时间：
2020-05-01
影响因子：
2.2
通讯作者：
Suzutani, Tatsuo
中科院分区：
文献类型：
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作者：
Kotani, Hideko;Koshizuka, Tetsuo;Suzutani, Tatsuo
人β-防御素2（HBD 2）是下生殖道的主要抗菌肽之一，在下生殖道中发挥着多种作用。基于Nugent评分作为细菌性阴道病的诊断标准，我们试图阐明Nugent评分与阴道分泌物中白细胞介素-6（IL-6）和HBD 2水平之间的相关性，并与各种类型的感染相关。本研究招募了98名女性。用酶联免疫吸附试验测定阴道洗液中HBD 2和IL-6的水平。根据Nugent方法，每个视野的乳酸杆菌形态型数量与HBD 2水平具有良好的相关性。HBD 2的量也与念珠菌属的存在密切相关。（P < 0.01）。体外实验表明，加入热灭活的C.白色念珠菌（HKCA）。HKCA的加入诱导Dectin-1 mRNA的表达。活化B细胞核因子κ轻链增强子（NF-κ B）反应元件的荧光素酶测定显示HKCA活化NF-κ B信号传导。这些结果表明C.白念珠菌诱导Dectin-1和（NF-κ B）信号传导的活化，导致HBD 2表达。总之，HBD 2的表达与乳酸杆菌和念珠菌的存在呈正相关。
As one of the main antimicrobial peptides, human beta-defensin 2 (HBD2) plays multiple roles in the lower genital tract. Based on the Nugent score as a diagnostic criterion for bacterial vaginosis, we sought to clarify the correlations among the Nugent score and interleukin-6 (IL-6) and HBD2 levels in vaginal secretions in association with various types of infection. Ninety-eight women were recruited for this study. Levels of HBD2 and IL-6 in vaginal wash were measured by enzyme-linked immunosorbent assays. According to the Nugent method, the number of Lactobacillus morphotypes per field of view was well correlated with the HBD2 level. The amount of HBD2 was also well correlated with the presence of Candida spp. (P < 0.01). In vitro experiments revealed that the expression of HBD2 from the human vaginal epithelial cell line, VK2/E6E7, was induced by the addition of heat-killed C. albicans (HKCA). The addition of HKCA induced expression of Dectin-1 mRNA. A luciferase assay for nuclear factor kappa-light-chain-enhancer of activated B cells (NF-kappa B) responsive elements showed that HKCA activated NF-kappa B signaling. These results suggested that C. albicans induced the activation of Dectin-1 and (NF-kappa B) signaling, resulting in HBD2 expression. In conclusion, the expression of HBD2 positively correlated with the presence of Lactobacillus and Candida spp.