Microelectrode Sensing of Adenosine/Adenosine-5′-triphosphate with Fast-Scan Cyclic Voltammetry
Microelectrode Sensing of Adenosine/Adenosine-5′-triphosphate with Fast-Scan Cyclic Voltammetry
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DOI：
10.1002/elan.200900559
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发表时间：
2010-06
期刊：
影响因子：
3
通讯作者：
Yida Xu;B. Venton
中科院分区：
文献类型：
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作者：
Yida Xu;B. Venton
腺苷和腺苷-5 ' -三磷酸（ATP）是重要的细胞外信号分子。在这里，我们使用快速扫描循环伏安法在碳纤维微电极上研究腺苷和ATP。虽然ATP和腺苷具有相似的氧化电位，但ATP的氧化电流高度依赖于缓冲液的pH和二价阳离子浓度，而腺苷的氧化电流则不是。因此，可以通过添加二价阳离子螯合剂或在不同pH值下校准电极来区分它们。在EDTA（乙二胺四乙酸酯）存在下，在腺苷和ATP的混合物中监测腺苷脱氨酶对腺苷的酶降解。这种传感方法在酶动力学或体外研究中很有前景。
Adenosine and adenosine-5′-triphosphate (ATP) are important extracellular signaling molecules. Here, we studied adenosine and ATP using fast-scan cyclic voltammetry at carbon-fiber microelectrodes. Although ATP and adenosine have similar oxidation potentials, ATP oxidation current was highly dependent on buffer pH and divalent cation concentrations but adenosine current was not. Therefore, they can be distinguished by adding a divalent cation chelator or calibrating electrodes at different pH values. The enzymatic degradation of adenosine by adenosine deaminase was monitored in a mixture of adenosine and ATP in presence of EDTA (ethylenediaminetetraacetate). This sensing method is promising for enzyme kinetics or in vitro studies.